Damage to DNA thymine residues in CHO cells by hydrogen peroxide and copper, ascorbate and copper, hypochlorite, or other oxidants: Protection by low MW polyethylene glycol

Conference · Mon Mar 11 00:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5695225

Schellenberg, K A; Shaeffer, J ^[1]

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1. Eastern Virginia Medical School, Norfolk (United States)

Polyethylene glycol (PEG) MW 200-600, has been shown to protect animals against oxidant and radiation damage. In order to study the mechanism the authors examined the effect of PEG on damage to thymine residues in the DNA of living Chinese hamster ovary (CHO) cells. After growing to confluence in the presence of (methyl{sup 3}H)thymidine, the cells were treated, usually for 1 hr, with various combinations of H{sub 2}O{sub 2}, Cu{sup ++}, Fe{sup ++}, Ocl{sup {minus}}, ascorbate UV or X-irradiation, and PEG MW 300. The oxidants H{sub 2}O{sub 2}/Cu{sup ++}, and OCL{sup {minus}} released {sup 3}H into the medium from DNA thymine, and also formed thymine glycol residues in the DNA that were assayed by alkaline borohydride. The presence of 10% PEG during treatment significantly reduced the release of {sup 3}H into the medium but did not prevent formation of thymine glycol residues bound to the DNA. PEG at 10% had no effect on the cloning efficiency of CHO cells.

• OSTI ID: 5695225
• Report Number(s): CONF-9104107-; CODEN: FAJOE
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:4; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CHO CELLS
SENSITIVITY
COPPER
TOXICITY
HYDROGEN PEROXIDE
POLYETHYLENE GLYCOLS
BIOLOGICAL EFFECTS
ASCORBIC ACID
CLONING
THYMIDINE
THYMINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
X RADIATION
ALCOHOLS
ANIMAL CELLS
AZINES
ELECTROMAGNETIC RADIATION
ELEMENTS
GLYCOLS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
IONIZING RADIATIONS
ISOTOPE APPLICATIONS
METALS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC POLYMERS
OXYGEN COMPOUNDS
PEROXIDES
POLYMERS
PYRIMIDINES
RADIATIONS
RIBOSIDES
TRANSITION ELEMENTS
URACILS
VITAMINS
560300* - Chemicals Metabolism & Toxicology
560120 - Radiation Effects on Biochemicals
Cells
& Tissue Culture