Combined submerged and solid substrate fermentation for the bioconversion of lignocellulose
A novel two-stage bioreactor has been designed for a combined submerged (SF) and solid substrate fermentation (SSF) of wheat straw. The straw was pretreated with steam, and cellulases from the culture fluid of Trichoderma reesei were adsorbed on it for increased bio-convertibility. SSF was conducted in the top part of the bioreactor by inoculating the straw with a 36-h mycelial culture of T. reesei, or Coriolus versicolor. In the bottom part of the fermenter, Endomycopsis fibuliger was grown in SF. The SF liquor was recirculated through the SSF stage at 24 hour intervals to remove glucose and other metabolites that may inhibit growth, and to maintain optimum moisture level and temperature. The removed glucose and other metabolites provided nutrients for the yeast in the SF stage. The combined fermentation resulted in overall higher biomass yield, increased bioconversion, increased cellulase production, and increased digestibility compared with single SSF or SF. (Refs. 16).
- Research Organization:
- Latvian Academy of Sciences, 226067 Riga, Kleisti, Latvian SSR, USSR. August Kirchenstein Institute of Microbiology; Colorado State Univ., Fort Collins, CO, 80523
- OSTI ID:
- 5692730
- Journal Information:
- Biotechnol. Bioeng.; (United States), Vol. 30:2
- Country of Publication:
- United States
- Language:
- English
Similar Records
Producing enzymes from molds to convert cellulose into glucose and alcohol. Final report
Enzymatic hydrolysis of various pretreated lignocellulosic substrates and the fermentation of the liberated sugars to ethanol and butanediol
Related Subjects
BIOREACTORS
DESIGN
RECYCLING
STRAW
BIOCONVERSION
FERMENTATION
WHEAT
ADSORPTION
BIOMASS
CELLULASE
CELLULOSE
CULTURE MEDIA
GLUCOSE
INHIBITION
INOCULATION
LIGNIN
METABOLITES
MOISTURE
MYCELIUM
NUTRIENTS
OPTIMIZATION
REMOVAL
STEAM
SUBSTRATES
TEMPERATURE CONTROL
TRICHODERMA VIRIDE
YIELDS
ALDEHYDES
BODY
CARBOHYDRATES
CEREALS
CONTROL
ENERGY SOURCES
ENZYMES
FUNGI
GLYCOSYL HYDROLASES
GRASS
HEXOSES
HYDROLASES
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PLANT TISSUES
PLANTS
POLYSACCHARIDES
RENEWABLE ENERGY SOURCES
SACCHARIDES
SORPTION
TISSUES
TRICHODERMA
140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989)