Radioimmunoassay for an inhibin-like peptide from human seminal plasma

Journal Article · Tue Jan 01 00:00:00 EST 1985 · J. Immunoassay; (United States)

DOI:https://doi.org/10.1080/01971528508063039· OSTI ID:5690506

Hammonds, Jr, R G; Li, C H; Yamashiro, D; Cabrera, C M; Westphal, M

Antisera raised in rabbits to a synthetic peptide consisting of 31 amino acids with a sequence identical to inhibin-like peptide (ILP) isolated from human seminal plasma afford a highly specific and sensitive radioimmunoassay. Synthetic ILP completely displaces antiserum binding of radioiodinated (Tyr4)-ILP, with half maximal displacement at 36 fmoles ILP/tube. ILP, (Tyr4)-ILP and ILP-(9-31) had essentially equal potency, while ILP-(1-25), ILP-(1-23) and ILP-(1-16) had reduced potency. No cross reactivity was found among a variety of peptide hormones and proteins. Human seminal plasma displaces 50% of (/sup 125/I-Tyr4)-ILP at dilutions equivalent to 50-250 pl/tube, corresponding to immunoreactive ILP concentration of 0.5-2.5 mg/ml.

• Research Organization: Univ. of California, San Francisco
• OSTI ID: 5690506
• Journal Information: J. Immunoassay; (United States), Vol. 4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PEPTIDES
RADIOIMMUNOASSAY
AMINO ACID SEQUENCE
IODINE 125
PEPTIDE HORMONES
PROTEINS
RABBITS
ANIMALS
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
HORMONES
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MOLECULAR STRUCTURE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques