Transfer of IncP plasmids to extremely acidophilic Thiobacillus thiooxidans

Journal Article · Wed Jan 01 00:00:00 EST 1992 · Applied and Environmental Microbiology; (United States)

OSTI ID:5688561

Jin, S M; Yan, W M; Wang, Z N ^[1]

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1. Shandong Univ. (China)

Thiobacillus thiooxidans is an acidophilic, obligately autotrophic bacterium which derives its energy by oxidizing reduced or partially reduced sulfur compounds and obtains its carbon by fixing carbon dioxide from the atmosphere. The strain is able to live in inorganic, acidic environments and is present in large numbers in coal mine drainage and in mineral ores. T. thiooxidans has been used industrially in metal leaching from mineral ores and in the microbial desulfurization of coal in combination with Thiobacillus ferrooxidans. Although T. thiooxidans has been well studied physiologically, very little is known about it genetics. The broad-host-range IncP plasmids RP4, R68.45, RP1::Tn501, and pUB307 were transferred directly to extremely acidophilic Thiobacillus thiooxidans from Escherichia coli by conjugation at frequencies of 10{sup {minus}5} to 10{sup {minus}7} per recipient. The ability of T. thiooxidans to receive and express the antibiotic resistance markers was examined. The plasmid RP4 was transferred back to E. coli from T. thiooxidans at a frequency of 1.0 {times} 10{sup {minus}3} per recipient.

• OSTI ID: 5688561
• Journal Information: Applied and Environmental Microbiology; (United States), Vol. 58:1; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
01 COAL, LIGNITE, AND PEAT
THIOBACILLUS OXIDANS
GENETIC ENGINEERING
ACID MINE DRAINAGE
DESULFURIZATION
ESCHERICHIA COLI
GENETICS
LEACHING
ORES
PLASMIDS
BACILLUS
BACTERIA
BIOLOGY
BIOTECHNOLOGY
CELL CONSTITUENTS
CHEMICAL REACTIONS
DISSOLUTION
MICROORGANISMS
SEPARATION PROCESSES
SULFUR-OXIDIZING BACTERIA
550200* - Biochemistry
010900 - Coal
Lignite
& Peat- Environmental Aspects