Fatty acid-binding protein in liver and small intestine of the preruminant calf

Journal Article · Wed Jan 01 00:00:00 EST 1986 · J. Dairy Sci.; (United States)

DOI:https://doi.org/10.3168/jds.S0022-0302(86)80380-0· OSTI ID:5687806

Jenkins, K J

Cytosol obtained from differential centrifugation of homogenates from liver and small intestine mucosa was incubated with 1-(/sup 14/C) oleic acid or 1-(/sup 14/C) palmitic acid and filtered through Sephadex G-75. Elution profiles for both tissues showed radioactivity in two main peaks, the first corresponding to binding of fatty acid to high molecular weight proteins and the second to a protein fraction with a molecular weight of approximately 12,000 daltons. The low molecular weight fraction had high fatty acid-binding activity, which was greater for oleic than palmitic acid. The findings demonstrate the presence of fatty acid-binding protein in liver and intestinal mucosa of the preruminant calf.

• Research Organization: Agricultural Canada, Ottawa, Ontario
• OSTI ID: 5687806
• Journal Information: J. Dairy Sci.; (United States), Vol. 69:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HEXADECANOIC ACID
BIOCHEMICAL REACTION KINETICS
OLEIC ACID
PROTEINS
CALVES
CARBON 14 COMPOUNDS
HOMOGENATES
LIVER
PHYSIOLOGY
SMALL INTESTINE
TRACER TECHNIQUES
ANIMALS
BODY
CARBOXYLIC ACIDS
CATTLE
DIGESTIVE SYSTEM
DOMESTIC ANIMALS
GASTROINTESTINAL TRACT
GLANDS
INTESTINES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
REACTION KINETICS
RUMINANTS
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques