Quantitative determination of islet cell surface antibodies using /sup 125/I-protein A

Journal Article · Sun May 01 00:00:00 EDT 1983 · Diabetes; (United States)

OSTI ID:5686776

Huen, A H; Haneda, M; Freedman, Z; Lernmark, A; Rubenstein, A H

A quantitative method to measure islet cell surface antibodies in human patients has been developed using /sup 125/I-protein A. Isolated, dispersed, viable rat islet cells prepared by collagenase digestion were fixed in 4% paraformaldehyde to allow storage for up to 7 wk at 4 degrees C. Human sera, heat inactivated and adsorbed with rat liver and kidney powder (100 mg/ml), were incubated with the fixed cells (50 x 10(3)) for 60 min at 37 degrees C. Thereafter the cells were washed and exposed to 5 x 10(5) cpm /sup 125/I-protein A, which binds to IgG attached to the cell surface. Assay precision (14%) and reproducibility (16%) were established by repeated analysis of pooled sera from healthy individuals and IDDM patients using pooled batches of islet cells. Using this method, islet cell surface antibodies were detected in 35% of insulin-dependent diabetic patients.

• Research Organization: Department of Medicine, University of Chicago, Illinois
• OSTI ID: 5686776
• Journal Information: Diabetes; (United States), Vol. 32:5
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
RADIOASSAY
PANCREAS
DIABETES MELLITUS
IODINE 125
PATIENTS
RATS
SOMATIC CELLS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE DISEASES
ENDOCRINE GLANDS
GLANDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
MAMMALS
METABOLIC DISEASES
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIOISOTOPES
RODENTS
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics