Effect of an 11. 5-hr/day exposure schedule on the distribution and toxicity of inhaled carbon tetrachloride in the rat
This study evaluated the differences in toxicity and tissue distribution for 16 groups of male rats repeatedly exposed to 100 ppm of /sup 14/CCl/sub 4/ vapors for 8 or 11.5 hr/day for periods of 1 to 10 days. Serum sorbitol dehydrogenase (SDH) was also evaluated for its sensitivity at detecting CCl/sub 4/-induced hepatotoxicity. Following 1, 2, 3, 4, 5, 7, 11, and 14 days, one group of rats from each exposure schedule was sacrificed and /sup 14/C activity in seven tissues and serum SDH levels were measured. To compare the effects of CCl/sub 4/ on the liver and kidney following repeated exposure to the two schedules, one group of rats was exposed for 8 hr/day for 10 of 12 consecutive days and another for 11.5 hr/day for 7 of 12 consecutive days so that each group received essentially the same dose (8000 ppm-hr) of CCl/sub 4/. The 11.5-hr/day exposure schedule, compared to rats exposed 8-hr/day, produced minor changes in the distribution and concentration of /sup 14/C (CCl/sub 4/ equivalents) in various tissues. Following 1 and 2 weeks of exposure to either schedule, the fat, liver, lungs, and adrenals had the highest concentration of CCl/sub 4/ equivalents. There were no significant differences in CCl/sub 4/-induced hepatotoxicity or nephrotoxicity between rats exposed to the two schedules following either 1 or 2 weeks of exposure as measured by histopathology. In contrast, rats exposed 11.5 hr/day had significantly higher SDH levels than those exposed to the 8-hr/day schedule; thus suggesting that the 11.5-hr schedule did produce a measurably greater degree of hepatotoxicity, although it was too subtle to detect pathologically. Rats exposed for a fourth and fifth day during the second week of the 11.5-hr schedule had a significantly greater concentration of /sup 14/C activity in the fat than rats exposed to the 8-hr/day schedule.
- Research Organization:
- Purdue Univ., West Lafayette, IN
- OSTI ID:
- 5686502
- Journal Information:
- Fundam. Appl. Toxicol.; (United States), Vol. 3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CARBON TETRACHLORIDE
TISSUE DISTRIBUTION
TOXICITY
OXIDOREDUCTASES
ENZYME ACTIVITY
ADRENAL GLANDS
BIOLOGICAL HALF-LIFE
CARBON 14 COMPOUNDS
FAT CELLS
INHALATION
KIDNEYS
LIVER
LUNGS
RATS
TRACER TECHNIQUES
ANIMAL CELLS
ANIMALS
BODY
CHLORINATED ALIPHATIC HYDROCARBONS
CONNECTIVE TISSUE CELLS
DIGESTIVE SYSTEM
DISTRIBUTION
ENDOCRINE GLANDS
ENZYMES
GLANDS
HALOGENATED ALIPHATIC HYDROCARBONS
INTAKE
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANS
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
550501 - Metabolism- Tracer Techniques