Ethanal assay, using an enzymo-conductimetric method
Journal Article
·
· Analytical Letters; (United States)
- I.C.B.M.C., Villeurbanne (France)
An enzymo-conductimetric method for the assay of ethanol is described. It is based on ethanol oxidation is presence of yeast aldehyde dehydrogenase. Experimental conditions compatible with conductimetry were optimized and kinetic parameters of the enzymatic reaction determined. Due to the low K{sub M} value of ethanol for the enzyme, an end-point assay was proposed. A linear relationship was demonstrated between experimental conductance changes and ethanol concentration up to 25 {mu}M. Validation of the assay was made on wines by comparison with a spectrophotometric method. Both methods gave similar results, but conductimetry avoids sample treatment if solutions are colored or turbid.
- OSTI ID:
- 5678258
- Journal Information:
- Analytical Letters; (United States), Vol. 25:1; ISSN 0003-2719
- Country of Publication:
- United States
- Language:
- English
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