Utilization of enzymatically hydrolyzed wood hemicelluloses by microorganisms for production of liquid fuels
Hemicellulose-derived sugars were obtained from a variety of pretreated wood substrates such as water-soluble fractions from steam-exploded aspen, solvent-extracted aspen, and commercial xylan. These fractions were enzymatically hydrolyzed by commercial enzyme preparations and by the culture filtrates of eight highly cellulolytic fungi. The sugars released were assayed by high-pressure liquid chromatography. Over 30% of the hemicellulose fractions, at a 10% substrate concentration, could be hydrolyzed to monosaccharides. These hemicellulose hydrolysates were used as the substrates for growth of Clostridium acetobutylicum and Klebsiella pneumoniae. Comparatively low butanol values were obtained with C. acetobutylicum, although over 50% of the hemicellulose fraction, at a 1% substrate concentration, could be converted to 2,3-butanediol, ethanol, and acetic acid by K. pneumoniae. (Refs. 25).
- Research Organization:
- Biotechnology and Chemistry Groups, Eastern Lab, Forintek Canada Corp, Ottawa, Ontario, Canada K1G 3Z5
- OSTI ID:
- 5676554
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Vol. 45:1
- Country of Publication:
- United States
- Language:
- English
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Enzymatic hydrolysis of various pretreated lignocellulosic substrates and the fermentation of the liberated sugars to ethanol and butanediol
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ACETIC ACID
PRODUCTION
BUTANEDIOLS
BUTANOLS
ETHANOL
HEMICELLULOSE
ENZYMATIC HYDROLYSIS
CLOSTRIDIUM ACETOBUTYLICUM
FERMENTATION
GROWTH
KLEBSIELLA
MONOSACCHARIDES
SACCHARIFICATION
WOOD
ALCOHOLS
BACTERIA
BIOCONVERSION
CARBOHYDRATES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CLOSTRIDIUM
DECOMPOSITION
GLYCOLS
HYDROLYSIS
HYDROXY COMPOUNDS
LYSIS
METHANOGENIC BACTERIA
MICROORGANISMS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
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