Method for the production of human T-T cell hybrids and production suppressor factor by human T-T cell hybrids

Patent · Tue Jun 27 00:00:00 EDT 1989

OSTI ID:5673759

Platsoucas, C

This patent describes a method for production of human T-T cell hybrids which produce Suppressor Factor wherein cells of lymphoid origin are fused with comprises: (a) mixing cells from a first parent cell line comprising a non-mutagenized Jurkat lymoblastoid T cell line, wherein the Jurkat lymphoblastoid cells are not sensitive and cannot be killed by hypoxanthine-aminopterin-thymidine medium, with a second parent cell comprising mitogen or alloantigen activated peripheral blood leukocyte T cells or purified T-cells, (b) allowing the first and second parent cells to fuse in the presence of polyethylene glycol for about 10-20 minutes with gentle agitation to generate hybrids in the cell mixture, (c) incubating the cell mixture containing the hybrids and the first and second parent cells, after removal of the polyethylene glycol, for periods of between one to sixty days at 37{sup 0} in 5% CO/sub 2/, (d) selecting for the hybrids by separating the hybrids from the first parent Jurkat lymphoblastoid cells by coloning in agar medium wherein the hybrids form colonies, (e) recovering the hybrids that form colonies in agar medium and expanding them in culture, and (f) determining the presence of Suppressor Factor in the culture and recovering the T-T cell hybrids which produce suppressor factor.

• Assignee: Sloan-Kettering Institute for Cancer Research, New York, NY
• Patent Number(s): US 4843004
• OSTI ID: 5673759
• Resource Relation: Patent File Date: 31 May 1984
• Country of Publication: United States
• Language: English

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LYMPHOCYTES
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PRODUCTION
ALCOHOLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
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HYDROXY COMPOUNDS
LEUKOCYTES
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