Mutagenic and lethal effects of near-ultraviolet radiation (290-400 nm) on bacteria and phage

Journal Article · Thu Jan 01 00:00:00 EST 1987 · Environ. Mol. Mutagen.; (United States)

DOI:https://doi.org/10.1002/em.2850100311· OSTI ID:5672848

Eisenstark, A

Despite decades of study of the effect of near-ultraviolet radiation (NUV) on bacterial cells, insights into mechanisms of deleterious alterations and subsequent recovery are just now emerging. These insights are based on observations that 1) damage by NUV may be caused by a reactive oxygen molecule, since H/sub 2/O/sub 2/ may be a photoproduct of NUV; 2) some, but not all, of the effects of NUV and H/sub 2/O/sub 2/ are interchangeable; 3) there is an inducible regulon (oxyR) that responds to oxidative stress and is involved in protection against NUV; 4) a number of NUV-sensitive mutants are defective either in the capacity to detoxify reactive oxygen molecules or to repair DNA damage caused by NUV; and 5) recovery from NUV damage may not directly involve induction of the SOS response. Since several distinctly different photoreceptors and targets are involved, it is unknown whether NUV lethality and mutagenesis result from an accumulation of damages or whether there is a particularly critical photoeffect. To fully understand the mechanisms involved, it is important to identify the chromophore(s) of NUV, the mechanism of toxic oxygen species generation, the role of the oxidative defense regulon (oxyR), the specific lesions in the DNA, and the enzymatic events of subsequent repair. 131 references.

• Research Organization: Univ. of Missouri, Columbia
• OSTI ID: 5672848
• Journal Information: Environ. Mol. Mutagen.; (United States), Vol. 10:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIOPHAGES
GENETIC RADIATION EFFECTS
ESCHERICHIA COLI
NEAR ULTRAVIOLET RADIATION
MUTAGENESIS
SALMONELLA TYPHIMURIUM
CATALASE
DNA REPAIR
HYDROGEN PEROXIDE
PORPHYRINS
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CARBOXYLIC ACIDS
ELECTROMAGNETIC RADIATION
ENZYMES
GENETIC EFFECTS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PARASITES
PEROXIDASES
PEROXIDES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SALMONELLA
ULTRAVIOLET RADIATION
VIRUSES
560130* - Radiation Effects on Microorganisms