DNA damage as a biological marker in aquatic organisms exposed to benzo(a)pyrene
We show that minute quantities of BaPDE-DNA adducts in the liver of bluegill sunfish can be detected and quantitated using a simple analytical technique whose sensitivity depends upon the intrinsic fluorescence of the specific adduct being analyzed. These adducts represent damage to DNA of the organism, which, if left uncorrected, could trigger a sequence of events that culminate in the appearance of an overt malignancy. We believe that the data reported here demonstrate that the covalent interaction of genotoxic chemicals with cellular macromolecules such as DNA is, potentially, a sensitive biological marker which could be of early predictive value in assessing exposure and its significance. 13 refs., 1 fig., 1 tab.
- Research Organization:
- Oak Ridge National Lab., TN (USA)
- DOE Contract Number:
- AC05-84OR21400
- OSTI ID:
- 5672374
- Report Number(s):
- CONF-870940-2; ON: DE88005436
- Resource Relation:
- Conference: 11. international symposium on polynuclear aromatic hydrocarbons, Gaithersburg, MD, USA, 23 Sep 1987; Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
BENZOPYRENE
DNA ADDUCTS
MUTAGENESIS
ACUTE EXPOSURE
CHRONIC EXPOSURE
FISHES
RISK ASSESSMENT
ADDUCTS
ANIMALS
AQUATIC ORGANISMS
AROMATICS
CONDENSED AROMATICS
HYDROCARBONS
ORGANIC COMPOUNDS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550200 - Biochemistry