Cytological effects of Tc on young soybean plants
- San Diego State Univ., CA (USA)
Soybean seedlings (Glycine max cv. Williams) were exposed for 24 to 67 h to {sup 99}TcO{sub 4}- (Tc) at various concentrations in dilute culture solution. Reduced primary leaf midrib length was observed with 67-h exposures to greater than or equal to 6.0 mu M Tc. Cellular effects were consistently observed by a light microscope after 43-h or longer exposure to 6.6 microM Tc and higher concentrations. At lower Tc levels, abnormal cells were interspersed among cells of normal appearance. Abnormal cells displayed blockshaped nuclei which were more densely stained by Harris' hematoxylineosin Y than controls; such cells frequently demonstrated incipient plasmolysis. The number of affected cells increased with dose; both nuclei and cytoplasm demonstrated greater staining intensity and more severe plasmolysis at higher levels. At levels of greater than or equal to 13.2 Tc, cellular damage was extensive. Cells were reduced in size and were highly plasmolysed; cell walls were distorted, and intercellular spaces were reduced or became nonexistent. Mitotic activity was observed at Tc levels less than or equal to 9.9 microM. Observed Tc cellular effects are attributed principally to the alteration of membrane permeability characteristics.
- OSTI ID:
- 5668568
- Journal Information:
- Health Physics; (USA), Vol. 57:2; ISSN 0017-9078
- Country of Publication:
- United States
- Language:
- English
Similar Records
Cell heterogeneity during the cell cycle
The Adaptive Response in p53 Cancer Prone Mice: Loss of heterozygosity and Genomic Instability
Related Subjects
CELL MEMBRANES
PERMEABILITY
GLYCINE HISPIDA
RADIONUCLIDE KINETICS
TECHNETIUM 99
TOXICITY
CELL NUCLEI
DOSE-RESPONSE RELATIONSHIPS
LEAVES
MITOSIS
PATHOLOGICAL CHANGES
SEEDLINGS
SOYBEANS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CONSTITUENTS
CELL DIVISION
FOOD
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LEGUMINOSAE
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
PLANTS
RADIOISOTOPES
TECHNETIUM ISOTOPES
VEGETABLES
YEARS LIVING RADIOISOTOPES
560162* - Radionuclide Effects
Kinetics
& Toxicology- Animals
Plants
Microorganisms
& Cells