Isolation of Clostridium thermocellum auxotrophs
The conversion of biomass of fuels and chemical feedstocks by microbial fermentation offers the potential of solving two of today's important problems: waste accumulation and exhaustion of fossil fuels. Microorganisms with the capabilities of converting biomass components such as cellulos and hemicellulose to chemicals and fuels in a single step are of particular interest. One such microorganism is Clostridium thermocellum, a thermophilic anaerobe which degrades cellulose to ethanol and organic acids. For efficient industrial use, the cellulolytic capacity of this strain must be improved by genetic means. Spontaneous and UV irradiation-induced auxotrophic mutants of Clostridium thermocellum, an anaerobic cellulolytic thermophile, were isolated after penicillin enrichment in a chemically defined medium.
- Research Organization:
- Massachusetts Inst. of Tech., Cambridge
- DOE Contract Number:
- EG-77-S-02-4198
- OSTI ID:
- 5664007
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Vol. 43:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CLOSTRIDIUM THERMOCELLUM
MUTATIONS
ANAEROBIC DIGESTION
BIODEGRADATION
BIOLOGICAL RADIATION EFFECTS
BIOMASS
CELLULOSE
FERMENTATION
GENETICS
MUTANTS
THERMOPHILIC CONDITIONS
ULTRAVIOLET RADIATION
BACTERIA
BIOCONVERSION
BIOLOGICAL EFFECTS
BIOLOGY
CARBOHYDRATES
CHEMICAL REACTIONS
CLOSTRIDIUM
DECOMPOSITION
DIGESTION
ELECTROMAGNETIC RADIATION
ENERGY SOURCES
MANAGEMENT
MICROORGANISMS
ORGANIC COMPOUNDS
POLYSACCHARIDES
PROCESSING
RADIATION EFFECTS
RADIATIONS
RENEWABLE ENERGY SOURCES
SACCHARIDES
WASTE MANAGEMENT
WASTE PROCESSING
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