Enrichment for CFU-C from murine and human bone marrow using soybean agglutinin
Mouse bone marrow and spleen cells agglutinated by soybean agglutinin (SBA) or peanut agglutinin (PNA) were previously shown to be enriched for spleen colony-forming cells (CFU-S) and sufficiently depleted of graft-versus-host reaction producing cells to allow hematologic reconstitution of lethally irradiated allogeneic recipient mice. A similar enrichment for cells capable of forming colonies in soft agar culture (CFU-C) has now been found in the SBA-agglutinated fraction of mouse bone marrow cells, in contrast to the finding that in human bone marrow the majority of the CFU-C are in the fraction not agglutinated by SBA. Cytofluorometric studies with fluorescein-labeled SBA (FITC-SBA) revealed that the majority of both mouse and human bone marrow cells bind the lectin. Experiments mixing the human marrow fractions separated by SBA reveal that true enrichment for CFU-C is achieved in the unagglutinated fraction, as opposed to a possible depletion of a suppressor cell population. Granulocytic, monocytic, and mixed cell colonies were all enriched in the SBA-unagglutinated cell fraction from human bone marrow.
- Research Organization:
- Memorial Sloan-Kettering Cancer Center, New York, NY
- OSTI ID:
- 5657807
- Journal Information:
- Blood; (United States), Vol. 59:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
IMMUNE REACTIONS
BIOLOGICAL RADIATION EFFECTS
AGGLUTININS
ANIMAL CELLS
BONE MARROW CELLS
COLONY FORMATION
FLUORESCENCE SPECTROSCOPY
GLYCINE HISPIDA
GRAFT-HOST REACTION
LETHAL IRRADIATION
MICE
SPLEEN CELLS
STEM CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL EFFECTS
CONNECTIVE TISSUE CELLS
EMISSION SPECTROSCOPY
IRRADIATION
LEGUMINOSAE
MAMMALS
PLANTS
RADIATION EFFECTS
RODENTS
SOMATIC CELLS
SPECTROSCOPY
VERTEBRATES
560152* - Radiation Effects on Animals- Animals