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Title: Steroid dynamics in the rabbit

Abstract

Male rabbits were infused at a constant rate with /sup 3/H-androstenedione//sup 14/C-estrone (n . 5) or /sup 3/H-testosterone//sup 14/C-estradiol-17 beta (n . 3) for 3 1/2 hr and blood samples were obtained over the last hour and analyzed for radioactivity as androstenedione (A), testosterone (T), estrone (E1), estradiol-17 beta (E2 beta) and estradiol-17 alpha (E2 alpha). The mean value for the metabolic clearance rate of androstenedione (MCRA) was 85 +/- 10 l/day/kg, which was significantly greater than the mean MCRE1 59 +/- 10 l/day/kg. MCRT, 42 +/- 8 l/day/kg, and MCRE2 beta, 45 +/- 9 l/day/kg were not different. The conversion ratio of androstenedione to testosterone (CRA,T) was greater than CRT,A but for the estrogens, CRE2 beta, E1 was greater than CRE1,E2 beta. CRE2 beta, E2 alpha was greater than CRE1,E2 alpha. The overall aromatization of androstenedione to estrone, the fraction of /sup 3/H-androstenedione infused into the blood and measured as /sup 3/H-estrone in blood (( rho)A,E1BB) was 0.0005 +/- 0.0001 and for (rho)T,E2 beta BB was 0.0012 +/- 0.0006. In the rabbit both sex hormone binding globulin (SHBG) and albumin binding may effect the MCRs, and peripheral aromatization of androgens occurs to a far lesser degree than in humansmore » and primates.« less

Authors:
; ;
Publication Date:
Research Org.:
Univ. of Massachusetts Medical Center, Worcester
OSTI Identifier:
5655724
Resource Type:
Journal Article
Journal Name:
Steroids; (United States)
Additional Journal Information:
Journal Volume: 43:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ANDROSTENEDIONE; METABOLISM; ESTRADIOL; ESTRONE; TESTOSTERONE; ALBUMINS; AROMATIZATION; BLOOD; CARBON 14 COMPOUNDS; CHEMICAL BONDS; CLEARANCE; GLOBULINS; RABBITS; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ANDROGENS; ANDROSTANES; ANIMALS; BIOLOGICAL MATERIALS; BODY FLUIDS; CHEMICAL REACTIONS; ESTRANES; ESTROGENS; HORMONES; HYDROXY COMPOUNDS; ISOTOPE APPLICATIONS; KETONES; LABELLED COMPOUNDS; MAMMALS; MATERIALS; ORGANIC COMPOUNDS; PROTEINS; STEROID HORMONES; STEROIDS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Bourget, C, Flood, C, and Longcope, C. Steroid dynamics in the rabbit. United States: N. p., 1984. Web. doi:10.1016/0039-128X(84)90041-2.
Bourget, C, Flood, C, & Longcope, C. Steroid dynamics in the rabbit. United States. https://doi.org/10.1016/0039-128X(84)90041-2
Bourget, C, Flood, C, and Longcope, C. 1984. "Steroid dynamics in the rabbit". United States. https://doi.org/10.1016/0039-128X(84)90041-2.
@article{osti_5655724,
title = {Steroid dynamics in the rabbit},
author = {Bourget, C and Flood, C and Longcope, C},
abstractNote = {Male rabbits were infused at a constant rate with /sup 3/H-androstenedione//sup 14/C-estrone (n . 5) or /sup 3/H-testosterone//sup 14/C-estradiol-17 beta (n . 3) for 3 1/2 hr and blood samples were obtained over the last hour and analyzed for radioactivity as androstenedione (A), testosterone (T), estrone (E1), estradiol-17 beta (E2 beta) and estradiol-17 alpha (E2 alpha). The mean value for the metabolic clearance rate of androstenedione (MCRA) was 85 +/- 10 l/day/kg, which was significantly greater than the mean MCRE1 59 +/- 10 l/day/kg. MCRT, 42 +/- 8 l/day/kg, and MCRE2 beta, 45 +/- 9 l/day/kg were not different. The conversion ratio of androstenedione to testosterone (CRA,T) was greater than CRT,A but for the estrogens, CRE2 beta, E1 was greater than CRE1,E2 beta. CRE2 beta, E2 alpha was greater than CRE1,E2 alpha. The overall aromatization of androstenedione to estrone, the fraction of /sup 3/H-androstenedione infused into the blood and measured as /sup 3/H-estrone in blood (( rho)A,E1BB) was 0.0005 +/- 0.0001 and for (rho)T,E2 beta BB was 0.0012 +/- 0.0006. In the rabbit both sex hormone binding globulin (SHBG) and albumin binding may effect the MCRs, and peripheral aromatization of androgens occurs to a far lesser degree than in humans and primates.},
doi = {10.1016/0039-128X(84)90041-2},
url = {https://www.osti.gov/biblio/5655724}, journal = {Steroids; (United States)},
number = ,
volume = 43:2,
place = {United States},
year = {Wed Feb 01 00:00:00 EST 1984},
month = {Wed Feb 01 00:00:00 EST 1984}
}