Inhibition of N-nitrosamine carcinogenesis and aflatoxin DNA damage by ellagic acid

Thesis/Dissertation · Fri Jan 01 00:00:00 EST 1988

OSTI ID:5645059

Mandal-Chaudhuri, S

The effect of ellagic acid (EA), on the tumorigenicity of N-nitrosobenzylmethylamine (NBMA) in the rat esophagus was investigated. Groups of 30 male F-344 rats were fed a semipurified diet containing EA for 27 weeks. N-nitrosobenzylmethylamine was administered subcutaneously, once a week for 18 weeks. Ellagic acid produced a significant inhibition in the average number of esophageal tumors at both 20 weeks and 27 weeks. To investigate the mechanism(s) of this inhibition, EA was tested for its effect on the metabolism, DNA-binding and DNA-adduct formation of NBMA in cultured explants of rat esophagus. Explants were incubated in medium containing EA at concentrations of 10, 50, and 100 {mu}M for 16 hours, followed by the addition of 1{mu}M ({sup 3}H)NBMA and EA for 12 hours. Explant DNA was isolated by phenol extraction and hydroxylapatite chromatography, and benzaldehyde formation was determined by h.p.l.c. analysis of the culture medium. Finally, EA was examined for its ability to inhibit DNA damage induced by aflatoxin B{sub 1} (AFB{sub 1}) in cultured explants of rat trachea and esophagus, and human tracheobronchus.

• Research Organization: Medical Coll. of Ohio, Toledo, OH (USA)
• OSTI ID: 5645059
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
AFLATOXIN
GENETIC EFFECTS
DNA ADDUCTS
BIOSYNTHESIS
ESOPHAGUS
NEOPLASMS
NITROSAMINES
CARCINOGENESIS
ORGANIC ACIDS
BIOLOGICAL EFFECTS
INHIBITION
LIQUID COLUMN CHROMATOGRAPHY
MAN
RATS
TOXICITY
TRACER TECHNIQUES
TRACHEA
TRITIUM COMPOUNDS
ADDUCTS
AMINES
ANIMALS
BODY
CHROMATOGRAPHY
DIGESTIVE SYSTEM
DISEASES
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MAMMALS
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOGENESIS
PRIMATES
RESPIRATORY SYSTEM
RODENTS
SEPARATION PROCESSES
SYNTHESIS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques