Differential expression of murine adult hemoglobins in early ontogeny
Abstract
A hemoglobin mutation is described that permits study of the expression of the two adult ..beta..-globin genes throughout fetal and postnatal development. Mice with a mutation at the Hbb/sup s/, ..beta..-globin locus, were used to study the relative levels of ..beta..-s2major and ..beta..-sminor globins specified by the mutant Hbb/sup s2/ haplotype during development. At 11.5 days of gestation ..beta..-sminor comprised over 80% and ..beta..-s2major under 20% of the adult beta-globin. The relative level of ..beta..-sminor decreased through fetal development; at birth ..beta..-sminor represented 33.7% of the ..beta..-globin. The adult values of 71.0% ..beta..-s2major and 29.0% ..beta..-sminor globin are expressed in mice six days after birth. Because the two ..beta..-globin genes are expressed in mice of the Hbb/sup 2s/ haplotype, both the ..beta..-smajor and ..beta..-sminor genes must be expressed in mice of the Hbb/sup s/ haplotype. Expression of the ..beta..-sminor gene is elevated to 35.6% in Hbb/sup s2/ mice that have been bled repeatedly. Thus, the 5' ..beta..-s2major and 3' ..beta..-sminor genes of the Hbb/sup s2/ haplotype and, presumably the 5' ..beta..-smajor and 3' ..beta..-sminor genes of the Hbb/sup s/ haplotype, are regulated independently and are homologous to the 5' ..beta..-dmajor and 3' ..beta..-dminor genes of the Hbb/sup d/ haplotype. Micemore »
- Authors:
- Publication Date:
- Research Org.:
- Tennessee Univ., Oak Ridge (USA). Graduate School of Biomedical Sciences; Research Triangle Inst., Research Triangle Park, NC (USA). Chemistry and Life Sciences Div.; Oak Ridge National Lab., TN (USA)
- OSTI Identifier:
- 5642932
- Report Number(s):
- CONF-8409198-1
ON: DE85005364
- DOE Contract Number:
- AC05-84OR21400
- Resource Type:
- Conference
- Resource Relation:
- Conference: Conference on hemoglobin switching, Airlie, VA, USA, 30 Sep 1984
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; BLOOD FORMATION; ONTOGENESIS; HEMOGLOBIN; GENES; QUANTITY RATIO; FETUSES; MICE; SICKLE CELL ANEMIA; THALASSEMIA; ANEMIAS; ANIMALS; CARBOXYLIC ACIDS; DISEASES; GLOBIN; HEMIC DISEASES; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; MAMMALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PIGMENTS; PORPHYRINS; PROTEINS; RODENTS; SYMPTOMS; VERTEBRATES; 550800* - Morphology
Citation Formats
Wawrzyniak, C J, Lewis, S E, and Popp, R A. Differential expression of murine adult hemoglobins in early ontogeny. United States: N. p., 1985.
Web.
Wawrzyniak, C J, Lewis, S E, & Popp, R A. Differential expression of murine adult hemoglobins in early ontogeny. United States.
Wawrzyniak, C J, Lewis, S E, and Popp, R A. 1985.
"Differential expression of murine adult hemoglobins in early ontogeny". United States.
@article{osti_5642932,
title = {Differential expression of murine adult hemoglobins in early ontogeny},
author = {Wawrzyniak, C J and Lewis, S E and Popp, R A},
abstractNote = {A hemoglobin mutation is described that permits study of the expression of the two adult ..beta..-globin genes throughout fetal and postnatal development. Mice with a mutation at the Hbb/sup s/, ..beta..-globin locus, were used to study the relative levels of ..beta..-s2major and ..beta..-sminor globins specified by the mutant Hbb/sup s2/ haplotype during development. At 11.5 days of gestation ..beta..-sminor comprised over 80% and ..beta..-s2major under 20% of the adult beta-globin. The relative level of ..beta..-sminor decreased through fetal development; at birth ..beta..-sminor represented 33.7% of the ..beta..-globin. The adult values of 71.0% ..beta..-s2major and 29.0% ..beta..-sminor globin are expressed in mice six days after birth. Because the two ..beta..-globin genes are expressed in mice of the Hbb/sup 2s/ haplotype, both the ..beta..-smajor and ..beta..-sminor genes must be expressed in mice of the Hbb/sup s/ haplotype. Expression of the ..beta..-sminor gene is elevated to 35.6% in Hbb/sup s2/ mice that have been bled repeatedly. Thus, the 5' ..beta..-s2major and 3' ..beta..-sminor genes of the Hbb/sup s2/ haplotype and, presumably the 5' ..beta..-smajor and 3' ..beta..-sminor genes of the Hbb/sup s/ haplotype, are regulated independently and are homologous to the 5' ..beta..-dmajor and 3' ..beta..-dminor genes of the Hbb/sup d/ haplotype. Mice of the Hbb/sup s2/ haplotype are better than mice of the Hbb/sup d/ haplotytpe for studying the mechanisms of hemoglobin switching because the Hbb/sup s2/ each of the three embryonic and two adult hemoglobins can be separated by electrophoresis. 17 refs., 3 figs.},
doi = {},
url = {https://www.osti.gov/biblio/5642932},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 1985},
month = {Tue Jan 01 00:00:00 EST 1985}
}