Presence of dynorphin-like immunoreactivity but not opiate binding in Walker-256 tumors

Journal Article · Mon Jul 15 00:00:00 EDT 1985 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(85)90418-7· OSTI ID:5642884

Bryant, H U; Conroy, W G; Isom, G E; Malven, P V; Yim, G K.W.

Walker-256 tumor tissue was removed from rats on day 8 of tumor growth. An acidified methanol extract of the tumor tissue was assayed for immunoreactive (ir) dynorphin-A 1-17 (DYN-17) and ir-dynorphin-A (DYN-8). Levels of ir-DYN-17 and ir-DYN-8 were nearly 4- and 8-fold higher, respectively, in tumors versus normal muscle. However, tumor homogenates did not exhibit specific /sup 3/H-naloxone binding. These results indicate that although the Walker-256 carcinosarcoma may produce opioids, it is unlikely that these ectopic substances have direct opioid actions on the tumor itself. 34 references, 1 figure.

• Research Organization: Purdue Univ., West Lafayette, IN
• OSTI ID: 5642884
• Journal Information: Life Sci.; (United States), Vol. 37:2
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
SARCOMAS
GROWTH
TUMOR CELLS
IMMUNE REACTIONS
CHEMICAL BONDS
HOMOGENATES
PEPTIDES
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMAL CELLS
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551001* - Physiological Systems- Tracer Techniques