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Title: Calcium: Some aspects of subcellular accumulation and distribution in milk

Abstract

Distribution and bioavailability of {sup 47}calcium in milk labeled by extrinsic and intrinsic methods was investigated. Milk from Sprague Dawley rats was labeled by both methods, and milk from a cow was labeled by the extrinsic method. Retention of {sup 47}Ca from milks administered to young male Sprague Dawley rats was determined through whole body counting for 6 days after administration of milk. Percent of {sup 47}Ca dose retained was 72% for extrinsically labeled cow milk, 62% for extrinsically labeled rat milk, and 55% for intrinsically labeled rat milk. Samples were fractionated by ultracentrifugation and by gel exclusion chromatography. {sup 47}Calcium distributions in rat milk labeled intrinsically or extrinsically were similar. The majority of {sup 47}Ca was found in a particulate, >30,000 molecular weight fraction. The amount of milk calcium retained by rats appeared to be related to the amount of noncasein micelle-associated calcium. When administered by intraperitoneal injection into rats, {sup 45}Ca specific activity of milk peaked in 60 to 90 minutes. In vitro {sup 45}Ca accumulation was compared in Golgi apparatus and endoplasmic reticulum from liver and mammary gland of lactating Dunkin Hartley guinea pigs. In the presence of ATP, highest accumulation per unit total fraction protein wasmore » found in Golgi apparatus (mammary gland 28% of available {sup 45}Ca, liver 11%) while 8% was accumulated by endoplasmic reticulum fractions.« less

Authors:
Publication Date:
Research Org.:
Virginia Polytechnic Inst. and State Univ., Blacksburg, VA (USA)
OSTI Identifier:
5642560
Resource Type:
Thesis/Dissertation
Resource Relation:
Other Information: Thesis (Ph. D.)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CALCIUM; SUBCELLULAR DISTRIBUTION; MILK; CHEMICAL COMPOSITION; ATP; BIOLOGICAL ACCUMULATION; BIOLOGICAL AVAILABILITY; CALCIUM 47; CATTLE; CHROMATOGRAPHY; ENDOPLASMIC RETICULUM; GUINEA PIGS; LACTATION; LIVER; MAMMARY GLANDS; RATS; TRACER TECHNIQUES; ULTRACENTRIFUGATION; ALKALINE EARTH ISOTOPES; ALKALINE EARTH METALS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BODY; BODY FLUIDS; CALCIUM ISOTOPES; CELL CONSTITUENTS; CENTRIFUGATION; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; DISTRIBUTION; DOMESTIC ANIMALS; ELEMENTS; EVEN-ODD NUCLEI; FOOD; GLANDS; INTERMEDIATE MASS NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; MATERIALS; METALS; NUCLEI; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANOIDS; ORGANS; RADIOISOTOPES; RODENTS; RUMINANTS; SEPARATION PROCESSES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Shappell, N W. Calcium: Some aspects of subcellular accumulation and distribution in milk. United States: N. p., 1989. Web.
Shappell, N W. Calcium: Some aspects of subcellular accumulation and distribution in milk. United States.
Shappell, N W. 1989. "Calcium: Some aspects of subcellular accumulation and distribution in milk". United States.
@article{osti_5642560,
title = {Calcium: Some aspects of subcellular accumulation and distribution in milk},
author = {Shappell, N W},
abstractNote = {Distribution and bioavailability of {sup 47}calcium in milk labeled by extrinsic and intrinsic methods was investigated. Milk from Sprague Dawley rats was labeled by both methods, and milk from a cow was labeled by the extrinsic method. Retention of {sup 47}Ca from milks administered to young male Sprague Dawley rats was determined through whole body counting for 6 days after administration of milk. Percent of {sup 47}Ca dose retained was 72% for extrinsically labeled cow milk, 62% for extrinsically labeled rat milk, and 55% for intrinsically labeled rat milk. Samples were fractionated by ultracentrifugation and by gel exclusion chromatography. {sup 47}Calcium distributions in rat milk labeled intrinsically or extrinsically were similar. The majority of {sup 47}Ca was found in a particulate, >30,000 molecular weight fraction. The amount of milk calcium retained by rats appeared to be related to the amount of noncasein micelle-associated calcium. When administered by intraperitoneal injection into rats, {sup 45}Ca specific activity of milk peaked in 60 to 90 minutes. In vitro {sup 45}Ca accumulation was compared in Golgi apparatus and endoplasmic reticulum from liver and mammary gland of lactating Dunkin Hartley guinea pigs. In the presence of ATP, highest accumulation per unit total fraction protein was found in Golgi apparatus (mammary gland 28% of available {sup 45}Ca, liver 11%) while 8% was accumulated by endoplasmic reticulum fractions.},
doi = {},
url = {https://www.osti.gov/biblio/5642560}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 1989},
month = {Sun Jan 01 00:00:00 EST 1989}
}

Thesis/Dissertation:
Other availability
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