Purification and reconstitution of chloride channels from kidney and trachea

Journal Article · Fri Jun 23 00:00:00 EDT 1989 · Science (Washington, D.C.); (USA)

DOI:https://doi.org/10.1126/science.2472007· OSTI ID:5641408

Landry, D W; Akabas, M H; Redhead, C; Edelman, A; Cragoe, Jr, E J; Al-Awqati, Q ^[1]

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1. Columbia Univ., New York, NY (USA)

Chloride channels mediate absorption and secretion of fluid in epithelia, and the regulation of these channels is now known to be defective in cystic fibrosis. Indanyl-oxyacetic acid 94 (IAA-94) is a high-affinity ligand for the chloride channel, and an affinity resin based on that structure was developed. Solubilized proteins from kidney and trachea membranes were applied to the affinity matrix, and four proteins with apparent molecular masses of 97, 64, 40, and 27 kilodaltons were eluted from the column by excess IAA-94. A potential-dependent {sup 36}Cl- uptake was observed after reconstituting these proteins into liposomes. Three types of chloride channels with single-channel conductances of 26, 100, and 400 picosiemens were observed after fusion of these liposomes with planar lipid bilayers. Similar types of chloride channels have been observed in epithelia.

• OSTI ID: 5641408
• Journal Information: Science (Washington, D.C.); (USA), Vol. 244:4911; ISSN 0036-8075
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHLORIDES
MEMBRANE TRANSPORT
PROTEINS
FRACTIONATION
CATTLE
CELL MEMBRANES
CHLORINE 36
CHROMATOGRAPHY
CONGENITAL DISEASES
ELECTROPHORESIS
ELECTROPHYSIOLOGY
EPITHELIUM
KIDNEYS
LIPOSOMES
MEMBRANE PROTEINS
MOLECULAR WEIGHT
RHODOPSIN
TRACER TECHNIQUES
TRACHEA
VALINOMYCIN
ANIMAL TISSUES
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CELL CONSTITUENTS
CHLORINE COMPOUNDS
CHLORINE ISOTOPES
DISEASES
DOMESTIC ANIMALS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HALIDES
HALOGEN COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MEMBRANES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PHYSIOLOGY
PIGMENTS
RADIOISOTOPES
RESPIRATORY SYSTEM
RUMINANTS
SEPARATION PROCESSES
TISSUES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
550201* - Biochemistry- Tracer Techniques
550901 - Pathology- Tracer Techniques