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Title: Ethanol production from enzymatic hydrolyzates of cellulosic fines and hemicellulose-rich liquors derived from aqueous/steam fractionation of forages

Abstract

This study was aimed at evaluating perennial forages (timothy grass, alfalfa, and reed canary grass) as substrates for ethanol production. Two fractions, derived from the aqueous/steam fractionation of these plants, were used as carbon sources for ethanol production: (1) a solution containing water-soluble hemicelluloses and (2) cellulosic fines recovered after a delignification step. Both fractions were enzymatically hydrolyzed. The hemicellulose-rich fraction was easily saccharified with 90% of theoretical yield. Cellulosic fines were saccharified at 60--70% of theoretical yield. Increasing the delignification of cellulosic fines by alkaline peroxide treatment resulted in higher sugar yields. The glucose-rich hydrolyzate from cellulosic fines was easily fermented to 80--90% of theoretical ethanol yield with Saccharomyces cerevisiae or Pachysolen tannophilus. The pentose-rich hydrolyzate from water-soluble hemicelluloses was fermented to only 20% of theoretical ethanol yield with Pachysolen tannophilus. A lime treatment of the hemicellulose-rich liquors improved cell growth but did not improve ethanol production.

Authors:
;  [1];  [2];  [3]
  1. Univ. Laval, Sainte-Foy, Quebec (Canada). Dept. of Food Science and Nutrition
  2. Agriculture and Agri-Food, Sainte-Foy, Quebec (Canada)
  3. Univ. de Sherbrooke, Quebec (Canada). Dept. of Chemical Engineering
Publication Date:
OSTI Identifier:
564139
Resource Type:
Journal Article
Journal Name:
Industrial and Engineering Chemistry Research
Additional Journal Information:
Journal Volume: 36; Journal Issue: 11; Other Information: PBD: Nov 1997
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; ETHANOL; BIOSYNTHESIS; CELLULOSE; HEMICELLULOSE; ENZYMATIC HYDROLYSIS; GRAMINEAE; ALFALFA; MATERIALS TESTING; DELIGNIFICATION; ALKALINE HYDROLYSIS; CHEMICAL REACTION YIELD

Citation Formats

Belkacemi, K, Turcotte, G, Savoie, P, and Chornet, E. Ethanol production from enzymatic hydrolyzates of cellulosic fines and hemicellulose-rich liquors derived from aqueous/steam fractionation of forages. United States: N. p., 1997. Web. doi:10.1021/ie970105j.
Belkacemi, K, Turcotte, G, Savoie, P, & Chornet, E. Ethanol production from enzymatic hydrolyzates of cellulosic fines and hemicellulose-rich liquors derived from aqueous/steam fractionation of forages. United States. https://doi.org/10.1021/ie970105j
Belkacemi, K, Turcotte, G, Savoie, P, and Chornet, E. 1997. "Ethanol production from enzymatic hydrolyzates of cellulosic fines and hemicellulose-rich liquors derived from aqueous/steam fractionation of forages". United States. https://doi.org/10.1021/ie970105j.
@article{osti_564139,
title = {Ethanol production from enzymatic hydrolyzates of cellulosic fines and hemicellulose-rich liquors derived from aqueous/steam fractionation of forages},
author = {Belkacemi, K and Turcotte, G and Savoie, P and Chornet, E},
abstractNote = {This study was aimed at evaluating perennial forages (timothy grass, alfalfa, and reed canary grass) as substrates for ethanol production. Two fractions, derived from the aqueous/steam fractionation of these plants, were used as carbon sources for ethanol production: (1) a solution containing water-soluble hemicelluloses and (2) cellulosic fines recovered after a delignification step. Both fractions were enzymatically hydrolyzed. The hemicellulose-rich fraction was easily saccharified with 90% of theoretical yield. Cellulosic fines were saccharified at 60--70% of theoretical yield. Increasing the delignification of cellulosic fines by alkaline peroxide treatment resulted in higher sugar yields. The glucose-rich hydrolyzate from cellulosic fines was easily fermented to 80--90% of theoretical ethanol yield with Saccharomyces cerevisiae or Pachysolen tannophilus. The pentose-rich hydrolyzate from water-soluble hemicelluloses was fermented to only 20% of theoretical ethanol yield with Pachysolen tannophilus. A lime treatment of the hemicellulose-rich liquors improved cell growth but did not improve ethanol production.},
doi = {10.1021/ie970105j},
url = {https://www.osti.gov/biblio/564139}, journal = {Industrial and Engineering Chemistry Research},
number = 11,
volume = 36,
place = {United States},
year = {Sat Nov 01 00:00:00 EST 1997},
month = {Sat Nov 01 00:00:00 EST 1997}
}