Complexity of the primary genetic response to mitogenic activation of human T cells
- National Inst. of Allergy and Infectious Diseases, Bethesda, MD (USA)
- National Cancer Inst., Bethesda, MD (USA)
The authors describe the isolation and characterization of more than 60 novel cDNA clones that constitute part of the immediate genetic response to resting human peripheral blood T cells after mitogen activation. This primary response was highly complex, both in the absolute number of inducible genes and in the diversity of regulation. Although most of the genes expressed in activated T cells were shared with the activation response of normal human fibroblasts, a significant number were more restricted in tissue specificity and thus likely encode or effect the differentiated functions of activated T cells. The activatable genes could be further differentiated on the basis of kinetics of induction, response to cycloheximide, and sensitivity to the immunosuppressive drug cylcosporin A. It is of note that cyclosporin A inhibited the expression of more than 10 inducible genes, which suggests that this drug has a broad genetic mechanism of action.
- OSTI ID:
- 5641168
- Journal Information:
- Molecular and Cellular Biology; (USA), Vol. 9:3; ISSN 0270-7306
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CELL DIFFERENTIATION
GENE REGULATION
LYMPHOCYTES
GENE AMPLIFICATION
MITOGENS
BIOLOGICAL EFFECTS
CLASSIFICATION
CYCLOHEXIMIDE
DNA-CLONING
FIBROBLASTS
IMMUNOSUPPRESSION
IMMUNOSUPPRESSIVE DRUGS
SENSITIVITY
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CLONING
CONNECTIVE TISSUE CELLS
DNA HYBRIDIZATION
DRUGS
FUNGICIDES
HYBRIDIZATION
LEUKOCYTES
MATERIALS
PESTICIDES
SOMATIC CELLS
550400* - Genetics
550300 - Cytology
560300 - Chemicals Metabolism & Toxicology