Stable isotope fractionation in photosynthesis: Analysis of autotrophic competence following transformation of the chloroplast genome of Chlamydomonas

Technical Report · Sat Jun 15 00:00:00 EDT 1991

OSTI ID:5640983

Boynton, J E; Gillham, N W; Osmond, C B

Isotopic techniques needed to assess the interactions between photosynthesis and respiration in Chlamydomonas have been devised for {sup 13}C, using plate and liquid cultures. The effectiveness of various transformation strategies for the chloroplast psbA gene has been evaluated with respect to their utility in constructing and characterizing strains homoplasmic for site-directed mutations in an otherwise isogenic background. Our analysis of the first site-directed change in the D-1 protein of Chlamydomonas indicates that a second site mutation (arg{sub 238} > lys) in the loop between transmembrane helices IV -- V can partially compensate for the reduced photosynthetic performance that accompanies the atrazine resistant mutation (ser{sub 264} > ala/gly) in this alga and in higher plants grown under high light intensities. 31 refs., 2 figs.

• Research Organization: Duke Univ., Durham, NC (United States)
• Sponsoring Organization: USDOE; USDOE, Washington, DC (United States)
• DOE Contract Number: FG05-89ER14005
• OSTI ID: 5640983
• Report Number(s): DOE/ER/14005-T1; ON: DE91015792
• Country of Publication: United States
• Language: English

Related Subjects

14 SOLAR ENERGY
59 BASIC BIOLOGICAL SCIENCES
CARBON 12
ISOTOPE RATIO
CARBON 13
CHLOROPLASTS
MUTAGENESIS
OXYGEN 18
ISOTOPE SEPARATION
CHLAMYDOMONAS
GENETIC ENGINEERING
PHOTOSYNTHETIC REACTION CENTERS
PROGRESS REPORT
ALGAE
CARBON ISOTOPES
CELL CONSTITUENTS
CHLOROPHYCOTA
DOCUMENT TYPES
EVEN-EVEN NUCLEI
EVEN-ODD NUCLEI
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
OXYGEN ISOTOPES
PLANTS
SEPARATION PROCESSES
STABLE ISOTOPES
UNICELLULAR ALGAE
140505* - Solar Energy Conversion- Photochemical
Photobiological
& Thermochemical Conversion- (1980-)
550200 - Biochemistry