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Title: Immunoscintigraphic detection of venous thrombosis of the lower extremities by means of human antifibrin monoclonal antibodies labeled with sup 111 In

Abstract

A new monoclonal antibody specific for the beta-chain of human fibrin (C22A) and labeled with 111In has been obtained and successfully used in rabbits and dogs for the in vivo detection of venous thrombosis. Studies in humans are currently ongoing. In order to assess the diagnostic value of 111In-antifibrin for the detection of venous thrombosis of the lower extremities, the authors investigated 25 consecutive patients. Ten patients had clinical and instrumental (contrast phlebography and duplex scanning) evidence of acute deep venous thrombosis (DVT), 3 had a long-standing DVT with relapsing episodes of swelling and pain, 5 had superficial venous thrombosis, and the remaining 7 had no signs of thrombosis at all. Twenty patients were being treated with heparin. All patients received 111In-antifibrin at the dose of 74 MBq IV and were scanned with a large field of view gamma camera coupled with a high-energy, parallel-hole collimator at 30 minutes and three, six, and twenty-four hours postinjection. Only the persistence of an abnormal uptake at twenty-four hours confirmed by two observers at visual inspection was considered as positive. A positive result was obtained in 9 of 10 DVT patients (90% sensitivity) and in all SVT patients. The single DVT patient withmore » a negative 111In-antifibrin test had the longest interval between scintigraphy and onset of symptoms (fifty-five days). Thus, the age of thrombi represented a substantial limitation for the test. A false-positive result was obtained in a single SVT patient, in whom also a deep involvement, unconfirmed by phlebography, was suspected (91.6% specificity).« less

Authors:
; ; ; ; ;  [1]
  1. Universita di Padova (Italy)
Publication Date:
OSTI Identifier:
5640662
Resource Type:
Journal Article
Journal Name:
Angiology; (USA)
Additional Journal Information:
Journal Volume: 40:7; Journal ID: ISSN 0003-3197
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; LEGS; RADIOIMMUNOSCINTIGRAPHY; THROMBOSIS; DIAGNOSIS; FIBRIN; INDIUM 111; MONOCLONAL ANTIBODIES; PATIENTS; SPECIFICITY; ANTIBODIES; BETA DECAY RADIOISOTOPES; BODY; BODY AREAS; CARDIOVASCULAR DISEASES; COAGULANTS; COUNTING TECHNIQUES; DAYS LIVING RADIOISOTOPES; DIAGNOSTIC TECHNIQUES; DISEASES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; HEMATOLOGIC AGENTS; HEMOSTATICS; INDIUM ISOTOPES; INTERMEDIATE MASS NUCLEI; ISOMERIC TRANSITION ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LIMBS; MINUTES LIVING RADIOISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PROTEINS; RADIOIMMUNODETECTION; RADIOISOTOPE SCANNING; RADIOISOTOPES; SCINTISCANNING; SCLEROPROTEINS; TRACER TECHNIQUES; VASCULAR DISEASES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics

Citation Formats

Lusiani, L, Zanco, P, Visona, A, Breggion, G, Pagnan, A, and Ferlin, G. Immunoscintigraphic detection of venous thrombosis of the lower extremities by means of human antifibrin monoclonal antibodies labeled with sup 111 In. United States: N. p., 1989. Web. doi:10.1177/000331978904000710.
Lusiani, L, Zanco, P, Visona, A, Breggion, G, Pagnan, A, & Ferlin, G. Immunoscintigraphic detection of venous thrombosis of the lower extremities by means of human antifibrin monoclonal antibodies labeled with sup 111 In. United States. https://doi.org/10.1177/000331978904000710
Lusiani, L, Zanco, P, Visona, A, Breggion, G, Pagnan, A, and Ferlin, G. 1989. "Immunoscintigraphic detection of venous thrombosis of the lower extremities by means of human antifibrin monoclonal antibodies labeled with sup 111 In". United States. https://doi.org/10.1177/000331978904000710.
@article{osti_5640662,
title = {Immunoscintigraphic detection of venous thrombosis of the lower extremities by means of human antifibrin monoclonal antibodies labeled with sup 111 In},
author = {Lusiani, L and Zanco, P and Visona, A and Breggion, G and Pagnan, A and Ferlin, G},
abstractNote = {A new monoclonal antibody specific for the beta-chain of human fibrin (C22A) and labeled with 111In has been obtained and successfully used in rabbits and dogs for the in vivo detection of venous thrombosis. Studies in humans are currently ongoing. In order to assess the diagnostic value of 111In-antifibrin for the detection of venous thrombosis of the lower extremities, the authors investigated 25 consecutive patients. Ten patients had clinical and instrumental (contrast phlebography and duplex scanning) evidence of acute deep venous thrombosis (DVT), 3 had a long-standing DVT with relapsing episodes of swelling and pain, 5 had superficial venous thrombosis, and the remaining 7 had no signs of thrombosis at all. Twenty patients were being treated with heparin. All patients received 111In-antifibrin at the dose of 74 MBq IV and were scanned with a large field of view gamma camera coupled with a high-energy, parallel-hole collimator at 30 minutes and three, six, and twenty-four hours postinjection. Only the persistence of an abnormal uptake at twenty-four hours confirmed by two observers at visual inspection was considered as positive. A positive result was obtained in 9 of 10 DVT patients (90% sensitivity) and in all SVT patients. The single DVT patient with a negative 111In-antifibrin test had the longest interval between scintigraphy and onset of symptoms (fifty-five days). Thus, the age of thrombi represented a substantial limitation for the test. A false-positive result was obtained in a single SVT patient, in whom also a deep involvement, unconfirmed by phlebography, was suspected (91.6% specificity).},
doi = {10.1177/000331978904000710},
url = {https://www.osti.gov/biblio/5640662}, journal = {Angiology; (USA)},
issn = {0003-3197},
number = ,
volume = 40:7,
place = {United States},
year = {Sat Jul 01 00:00:00 EDT 1989},
month = {Sat Jul 01 00:00:00 EDT 1989}
}