Accumulation of deuterium oxide in body fluids after ingestion of D/sub 2/O-labeled beverages
A simple low-cost procedure was developed to compare the temporal profiles of deuterium oxide (D/sub 2/O) accumulation in body fluids after ingestion of D/sub 2/O-labeled solutions. D/sub 2/O concentration was measured in plasma and saliva samples taken at various intervals after ingestion of 20 ml of D/sub 2/O mixed with five solutions differing in carbohydrate and electrolyte concentrations. An infrared spectrometer was used to measure D/sub 2/O in purified samples obtained after a 48-h incubation period during which the water (D/sub 2/O and H/sub 2/O) in the sample was equilibrated with an equal volume of distilled water in a sealed diffusion dish. The procedure yields 100% recoveries of 60-500 ppm D/sub 2/O with an average precision of 5%. When compared with values for distilled water, D/sub 2/O accumulation in serial samples of plasma and saliva was slower for ingested solutions containing 40 and 15% glucose and faster for hypotonic saline and a 6% carbohydrate-electrolyte solution. These differences appear to reflect known differences in gastric emptying and intestinal absorption of these beverages. Therefore, this technique may provide a useful index of the rate of water uptake from ingested beverages into the body fluids.
- Research Organization:
- Univ. of South Carolina, Columbia
- OSTI ID:
- 5640311
- Journal Information:
- J. Appl. Physiol.; (United States), Vol. 63:5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BEVERAGES
INGESTION
BODY FLUIDS
CHEMICAL COMPOSITION
WATER
INTESTINAL ABSORPTION
CARBOHYDRATES
DEUTERIUM
ELECTROLYTES
GLUCOSE
INFRARED SPECTRA
LABELLED COMPOUNDS
SALIVA
ABSORPTION
ALDEHYDES
BIOLOGICAL MATERIALS
FOOD
HEXOSES
HYDROGEN COMPOUNDS
HYDROGEN ISOTOPES
INTAKE
ISOTOPES
LIGHT NUCLEI
MATERIALS
MONOSACCHARIDES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
SACCHARIDES
SPECTRA
STABLE ISOTOPES
UPTAKE
550201* - Biochemistry- Tracer Techniques