Alteration of gene expression during the induction of freezing tolerance in Brassica napus suspension cultures

Journal Article · Sun Nov 01 00:00:00 EST 1987 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.85.3.699· OSTI ID:5640264

Johnson-Flanagan, A M; Singh, J

Brassica napus suspension-cultured cells can be hardened to a lethal temperature for 50% of the sample of -20/sup 0/C in eight days at room temperature with abscisic acid. During the induction of freezing tolerance, changes were observed in the electrophoretic pattern of (/sup 35/S)methionine labeled polypeptides. In hardening cells, a 20 kilodalton polypeptide was induced on day 2 and its level increased during hardening. The induction of freezing tolerance with nonmaximal hardening regimens also resulted in increases in the 20 kilodalton polypeptide. The 20 kilodalton polypeptide was associated with a membrane fraction enriched in endoplasmic reticulum and was resolved as a single spot by two-dimensional electrophoresis. In vitro translation of mRNA indicate alteration of gene expression during abscisic acid induction of freezing tolerance. The new mRNA encodes a 20 kilodalton polypeptide associated with increased freezing tolerance induced by either abscisic acid or high sucrose. A 20 kilodalton polypeptide was also translated by mRNA isolated from cold-hardened B. napus plants.

• Research Organization: Agriculture Canada, Ottawa, Ontario
• OSTI ID: 5640264
• Journal Information: Plant Physiol.; (United States), Vol. 85:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BRASSICA
FREEZING
GENE REGULATION
POLYPEPTIDES
BIOSYNTHESIS
SUBCELLULAR DISTRIBUTION
ABSCISIC ACID
CELL CULTURES
ENDOPLASMIC RETICULUM
MESSENGER-RNA
METHIONINE
SULFUR 35
TRACER TECHNIQUES
TWO-DIMENSIONAL ELECTROPHORESIS
AMINO ACIDS
AUXINS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
DRUGS
ELECTROPHORESIS
EVEN-ODD NUCLEI
FOOD
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MONOCARBOXYLIC ACIDS
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANOIDS
PEPTIDES
PLANT GROWTH REGULATORS
PLANTS
PROTEINS
RADIOISOTOPES
RNA
SULFUR ISOTOPES
SYNTHESIS
VEGETABLES
550201* - Biochemistry- Tracer Techniques