Primary structure of the human melanoma-associated antigen p97 (melanotransferrin) deduced from the mRNA sequence

Journal Article · Sat Mar 01 00:00:00 EST 1986 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.83.5.1261· OSTI ID:5639557

Rose, T M; Plowman, G D; Teplow, D B; Dreyer, W J; Hellstroem, K E; Brown, J P

p97 is a cell-surface glycoprotein that is present in most human melanomas but only in trace amounts in normal adult tissues. To determine the structure of this tumor-associated antigen and to identify its functional domains, the authors have purified and cloned p97 mRNA and determined its nucleotide sequence. The mRNA encodes a 738-residue precursor, which contains the previously determined N-terminal amino acid sequence of p97. After removal of a 19-residue signal peptide, the mature p97 molecule comprises extracellular domains of 342 and 352 residues and a C-terminal 25-residue stretch of predominantly uncharged and hydrophobic amino acids, which we believe acts as a membrane anchor. Each extracellular domain contains 14 cysteine residues, which form seven intradomain disulfide bridges, and one or two potential N-glycosylation sites. Protease digestion studies show that the three major antigenic determinants of p97 are present on the N-terminal domain. The domains are strikingly homologous to each other (46% amino acid sequence homology) and to the corresponding domains of human serum transferrin (39% homology). Conservation of disulfide bridges and of amino acids thought to compose the iron binding pockets suggests that p97 is also related to transferrin in tertiary structure and function. They propose that p97 be renamed melanotransferrin to denote its original identification in melanoma cells and its evolutionary relationship to serotransferrin and lactotransferrin, the other members of the transferrin superfamily.

• Research Organization: ONCOGEN, Seattle, WA
• OSTI ID: 5639557
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 83:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANTIGENS
MOLECULAR STRUCTURE
RECOMBINANT DNA
LABELLING
DNA SEQUENCING
GLUCOPROTEINS
MAN
MELANOMAS
MESSENGER-RNA
OLIGONUCLEOTIDES
PHOSPHORUS 32
TRANSFERRIN
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
DAYS LIVING RADIOISOTOPES
DISEASES
DNA
GLOBULINS
GLOBULINS-BETA
ISOTOPES
LIGHT NUCLEI
MAMMALS
METALLOPROTEINS
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
RNA
SACCHARIDES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques