Mapping of odor-related neuronal activity in the olfactory bulb by high-resolution 2-deoxyglucose autoradiography
The spatial distribution of odor-induced neuronal activity in the olfactory bulb, the first relay station of the olfactory pathway, is believed to reflect important aspects of chemosensory coding. We report here the application of high-resolution 2-deoxyglucose autoradiography to the mapping of spatial patterns of metabolic activity at the level of single neurons in the olfactory bulb. It was found that glomeruli, which are synaptic complexes containing the first synaptic relay, tend to be uniformly active or inactive during odor exposure. Differential 2-deoxyglucose uptake was also observed in the somata of projection neurons (mitral cells) and interneurons (periglomerular and granule cells). This confirms and extends our previous studies in which odor-specific laminar and focal uptake patterns were revealed by the conventional x-ray film 2-deoxyglucose method due to Sokoloff and colleagues (Sokoloff, L., Reivich, M., Kennedy, C., DesRosiers, M. H., Patlak, C. S., Pettigrew, K. D., Sakurada, O. and Shinohara, M. (1977) J. Neurochem. 28, 897-916). Based on results obtained by the two methods, it is suggested that the glomerulus as a whole serves as a functional unit of activity. The high-resolution results are interpreted in terms of the well-characterized synaptic organization of the olfactory bulb and also serve to illustrate the capability of the 2-deoxyglucose autoradiographic technique to map metabolic activity in single neurons of the vertebrate central nervous system.
- Research Organization:
- Yale Univ. School of Medicine, New Haven, CT
- OSTI ID:
- 5637955
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 79:2
- Country of Publication:
- United States
- Language:
- English
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NERVE CELLS
PHYSIOLOGY
OLFACTORY BULBS
AUTORADIOGRAPHY
BIOLOGICAL FUNCTIONS
CARBON 14 COMPOUNDS
GLOMERULI
GLUCOSE
LABELLED COMPOUNDS
MEASURING METHODS
RATS
REDUCTION
ALDEHYDES
ANIMAL CELLS
ANIMALS
BODY
BRAIN
CARBOHYDRATES
CENTRAL NERVOUS SYSTEM
CHEMICAL REACTIONS
FUNCTIONS
HEXOSES
KIDNEYS
MAMMALS
MONOSACCHARIDES
NERVOUS SYSTEM
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VERTEBRATES
551001* - Physiological Systems- Tracer Techniques