A vaccine prepared from the 22 nm particles of surface hepatitis B antigen (HBsAg)
A method for obtaining a subunit inactivated vaccine preparation from the 22-nm particles of HBsAg is proposed. For inactivation of the residual infectious hepatitis B virus (HBV) the preparations were successively treated with 1% sodium dodecyl sulfate (SDS) and nucleases. In addition, thermal denaturation and ultraviolet irradiation of HBV DNA were used. As a control the biologic activity of a reference virus (SV40) was tested after the same treatment. The effectiveness of DNA inactivation was monitored by adding 3H-thymidine labeled reference virus to the vaccine preparations. The purified and inactivated HBsAg was adsorbed on Al2O3. Antigenicity was calculated on the basis of the determination of antibody in guinea pigs immunized with various doses of the vaccine, and the release of /sup 125/I- HBsAg from blood and kidneys in immunized and control mice was analyzed. Possible methods of inactivation and control of HBV vaccine is discussed.
- OSTI ID:
- 5636790
- Journal Information:
- J. Med. Virol.; (United States), Vol. 5:4
- Country of Publication:
- United States
- Language:
- English
Similar Records
A Combination of Human Broadly Neutralizing Antibodies against Hepatitis B Virus HBsAg with Distinct Epitopes Suppresses Escape Mutations
Construction and immunological evaluation of truncated hepatitis B core particles carrying HBsAg amino acids 119–152 in the major immunodominant region (MIR)
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
VACCINES
CHEMICAL PREPARATION
VIRUSES
BIOLOGICAL RADIATION EFFECTS
ANTIGENS
BLOOD
DNA
IMMUNOLOGY
INFECTIOUS HEPATITIS
IODINE 125
KIDNEYS
LABELLING
MICE
THERMAL DEGRADATION
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTROMAGNETIC RADIATION
ELECTRON CAPTURE RADIOISOTOPES
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PARASITES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RODENTS
SYNTHESIS
VERTEBRATES
VIRAL DISEASES
560134* - Radiation Effects on Microorganisms- Vaccine Preparation & Other Applications- (-1987)
550701 - Microbiology- Tracer Techniques
550201 - Biochemistry- Tracer Techniques