Fluorometric reaction rate method for determination of hydrogen perioxide at the nanomolar level

Journal Article · Tue Jul 01 00:00:00 EDT 1986 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00121a028· OSTI ID:5630707

Peinado, J; Torbio, F; Perez-Bendito, D

A kinetic nonenzymatic method is proposed for determination of hydrogen peroxide at the nanomolar level and applied to the analysis of several types of coffee, tea, and milk samples. The method is based on the Mn(II)-catalyzed oxidation of 2-hydroxynaphthaldehyde thiosemicarbazone (HNTS) by H/sub 2/O/sub 2/. The reaction is monitored spectrofluorometrically by measuring the initial rate at the excitation and emission wavelength of the oxidation product. The kinetic study involved obtaining the rate law and evaluating and optimizing the chemical variables. Sallent features of the method are (a) a linear calibration graph is obtained from 50 to 2000 nM; (b) no enzymes are required; and (c) it tolerates moderate amounts of various metal ions.

• Research Organization: Univ. of Cordoba, Spain
• OSTI ID: 5630707
• Journal Information: Anal. Chem.; (United States), Vol. 58:8
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
ALDEHYDES
REDOX REACTIONS
BEVERAGES
QUANTITATIVE CHEMICAL ANALYSIS
CARBAZONES
HYDROGEN PEROXIDE
MANGANESE
CATALYTIC EFFECTS
MILK
CATALYSIS
CHEMICAL REACTION KINETICS
EXPERIMENTAL DATA
FLUORESCENCE
FLUORESCENCE SPECTROSCOPY
TRACE AMOUNTS
BIOLOGICAL MATERIALS
BODY FLUIDS
CARBONIC ACID DERIVATIVES
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
DATA
ELEMENTS
EMISSION SPECTROSCOPY
FOOD
HYDROGEN COMPOUNDS
INFORMATION
KINETICS
LUMINESCENCE
MATERIALS
METALS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PEROXIDES
REACTION KINETICS
SPECTROSCOPY
TRANSITION ELEMENTS
400104* - Spectral Procedures- (-1987)
400301 - Organic Chemistry- Chemical & Physicochemical Properties- (-1987)