High-performance liquid chromatographic separation of biologically important arsenic species utilizing on-line inductively coupled argon plasma atomic emission spectrometric detection

Journal Article · Sun Jun 01 00:00:00 EDT 1986 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00298a015· OSTI ID:5630404

Spall, W D; Lynn, J G; Andersen, J L; Valdez, J G; Gurley, L R

An anion exchange, high-performance liquid chromatography technique using a 15-min linear gradient from water to 0.5 M ammonium carbonate to separate arsenite, arsenate, methylarsonic acid, and dimethylarsinic acid from neutral arsenic containing compounds was developed for application to a study of arsenic metabolism in cultured cell suspensions. Arsenic detection was accomplished by the direct coupling of the column effluent to an inductively coupled argon plasma atomic emission spectrometer (ICAP-AES) set to monitor the arsenic emission line at 197.19 nm. The analysis requires 20 min and is sensitive to as low as 60 ng of arsenic injected to the column.

• Research Organization: Los Alamos National Lab., NM
• DOE Contract Number: W-7405-ENG-36
• OSTI ID: 5630404
• Journal Information: Anal. Chem.; (United States), Vol. 58:7
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
ARSENATES
EMISSION SPECTROSCOPY
LIQUID COLUMN CHROMATOGRAPHY
ARSENIC COMPOUNDS
ARSONIC ACIDS
BIOLOGICAL MATERIALS
QUANTITATIVE CHEMICAL ANALYSIS
ORGANIC ACIDS
AMMONIUM CARBONATES
ANIONS
ARGON
EXPERIMENTAL DATA
ION EXCHANGE
PLASMA
TRACE AMOUNTS
AMMONIUM COMPOUNDS
CARBON COMPOUNDS
CARBONATES
CHARGED PARTICLES
CHEMICAL ANALYSIS
CHROMATOGRAPHY
DATA
ELEMENTS
FLUIDS
GASES
INFORMATION
IONS
MATERIALS
NONMETALS
NUMERICAL DATA
ORGANIC ARSENIC COMPOUNDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
RARE GASES
SEPARATION PROCESSES
SPECTROSCOPY
400105* - Separation Procedures
400104 - Spectral Procedures- (-1987)