Phosphorylation in vitro of human fibrinogen with casein kinase TS and characterization of phosphorylated sites
Human fibrinogen was phosphorylated by casein kinase TS. The (/sup 32/P)phosphate incorporated varied between 0.5 and 1 mol of phosphate per mole of fibrinogen. The phosphate was localized to Ser523 and Ser590 and serine and threonine residues between amino acids 259 and 268 in the A alpha-chain. In addition, Thr416 and Ser420 were phosphorylated in the gamma'-chain, which is a variant of the gamma-chain, constituting 7-10% of the gamma-chain population. The functional significance of casein kinase TS-induced phosphorylation of fibrinogen remains unknown; however, a slight but consistent increase of the turbidity in a gelation assay was observed for phosphorylated compared to unphosphorylated fibrinogen.
- Research Organization:
- Biomedical Center, Uppsala, Sweden
- OSTI ID:
- 5630148
- Journal Information:
- Arch. Biochem. Biophys.; (United States), Vol. 257:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
FIBRINOGEN
PHOSPHORYLATION
PHOSPHOTRANSFERASES
ENZYME ACTIVITY
CHYMOTRYPSIN
IN VITRO
MAN
MOLECULAR WEIGHT
PHOSPHATES
PHOSPHORUS 32
TRACER TECHNIQUES
TRYPSIN
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD COAGULATION FACTORS
CHEMICAL REACTIONS
COAGULANTS
DAYS LIVING RADIOISOTOPES
DRUGS
ENZYMES
GLOBULINS
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDE HYDROLASES
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PRIMATES
PROTEINS
RADIOISOTOPES
SERINE PROTEINASES
TRANSFERASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques