Sucrose accumulation in mature sweet melon fruits. [Cucumis melo]
Mesocarp tissue from sucrose-accumulating sweet melon (Cucumis melo cv. Galia) showed sucrose synthase activity (ca 1 nkat/gfw) while soluble acid invertase and sucrose phosphate synthase activities were not observed. Sucrose uptake into mesocarp discs was linear with sucrose concentration (1-500 mM) and unaffected by PCMBS and CCCP. Sucrose compartmentation into the vacuole also increased linearly with sucrose concentration as indicated by compartmental efflux kinetics. Mesocarp discs incubated in /sup 14/C-fructose + UDP-glu synthesized /sup 14/C-sucrose and efflux kinetics indicated that the /sup 14/C-sucrose was compartmentalized. These data support the hypothesis that two mechanisms are involved in sucrose accumulation in sweet melon: (1) compartmentation of intact sucrose and (2) synthesis of sucrose via sucrose synthase and subsequent compartmentation in the vacuole.
- Research Organization:
- ARO, Bet-Dagan, Israel
- OSTI ID:
- 5629768
- Report Number(s):
- CONF-8707108-; TRN: 88-001674
- Journal Information:
- Plant Physiol., Suppl.; (United States), Vol. 83:4; Conference: Annual meeting of the American Society of Plant Physiologists, St. Louis, MO, USA, 19 Jul 1987
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
FRUITS
ENZYME ACTIVITY
SACCHAROSE
BIOLOGICAL LOCALIZATION
BIOSYNTHESIS
CARBON 14 COMPOUNDS
TRACER TECHNIQUES
CARBOHYDRATES
DISACCHARIDES
FOOD
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
SACCHARIDES
SYNTHESIS
550201* - Biochemistry- Tracer Techniques