Sucrose accumulation in mature sweet melon fruits. [Cucumis melo]

Conference · Wed Apr 01 00:00:00 EST 1987 · Plant Physiol., Suppl.; (United States)

OSTI ID:5629768

Schaffer, A A; Aloni, B

Mesocarp tissue from sucrose-accumulating sweet melon (Cucumis melo cv. Galia) showed sucrose synthase activity (ca 1 nkat/gfw) while soluble acid invertase and sucrose phosphate synthase activities were not observed. Sucrose uptake into mesocarp discs was linear with sucrose concentration (1-500 mM) and unaffected by PCMBS and CCCP. Sucrose compartmentation into the vacuole also increased linearly with sucrose concentration as indicated by compartmental efflux kinetics. Mesocarp discs incubated in /sup 14/C-fructose + UDP-glu synthesized /sup 14/C-sucrose and efflux kinetics indicated that the /sup 14/C-sucrose was compartmentalized. These data support the hypothesis that two mechanisms are involved in sucrose accumulation in sweet melon: (1) compartmentation of intact sucrose and (2) synthesis of sucrose via sucrose synthase and subsequent compartmentation in the vacuole.

• Research Organization: ARO, Bet-Dagan, Israel
• OSTI ID: 5629768
• Report Number(s): CONF-8707108-; TRN: 88-001674
• Journal Information: Plant Physiol., Suppl.; (United States), Vol. 83:4; Conference: Annual meeting of the American Society of Plant Physiologists, St. Louis, MO, USA, 19 Jul 1987
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
FRUITS
ENZYME ACTIVITY
SACCHAROSE
BIOLOGICAL LOCALIZATION
BIOSYNTHESIS
CARBON 14 COMPOUNDS
TRACER TECHNIQUES
CARBOHYDRATES
DISACCHARIDES
FOOD
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
SACCHARIDES
SYNTHESIS
550201* - Biochemistry- Tracer Techniques