Glucocorticoid receptors in murine erythroleukaemic cells

Journal Article · Thu Jan 01 00:00:00 EST 1987 · J. Receptor Res.; (United States)

DOI:https://doi.org/10.3109/10799898709056778· OSTI ID:5629710

Hammond, K D; Torrance, J M; DiDomenico, M

Glucocorticoid receptors in murine erythroleukaemic cells were studied in relation to hexamethylene bisacetamide (HMBA) induced differentiation. Specific binding of dexamethasone was measured. A single class of saturable, high affinity binding sites was demonstrated in intact cells; with cell homogenates or fractions binding was low and could not be reliably quantified. Receptor binding in whole cell suspensions was lower in cells which had been treated with HMBA (36.5 +/- 8.2 pmol/g protein) than in untreated controls (87.9 +/- 23.6 pmol/g protein); dissociation constants were similar in treated (2.7 nM) and untreated cells (2.5 nM). Dexamethasone, hydrocortisone, corticosterone and progesterone competed with tritium-labelled dexamethasone for receptor binding sites; cortisone, deoxycorticosterone and oestradiol had little effect.

• Research Organization: Univ. of the Witwatersrand Medical School, Johannesburg, South Africa
• OSTI ID: 5629710
• Journal Information: J. Receptor Res.; (United States), Vol. 7:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DEXAMETHASONE
BIOCHEMICAL REACTION KINETICS
RECEPTORS
TUMOR CELLS
CELL DIFFERENTIATION
LEUKEMIA
MICE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ADRENAL HORMONES
ANIMAL CELLS
ANIMALS
CORTICOSTEROIDS
DISEASES
GLUCOCORTICOIDS
HEMIC DISEASES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
NEOPLASMS
ORGANIC COMPOUNDS
PREGNANES
PROTEINS
REACTION KINETICS
RODENTS
STEROIDS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques