Immunoglobulins acquire the ability to interact with DNA after chromatography on QAE-Sephadex
It was established that IgG isolated from the sera of healthy humans contains a substantial number of antibodies that react with native DNA. Their ability to interact with DNA is manifested only after chromatography on an anion exchange resin, as a result of which IgG is divided into two portions - acid and basic immunoglobulins. The peculiarities of the interaction of both fractions with DNA and the specificity of this reaction were investigated. It was shown that the investigated IgG can react with native and denatured DNA, dextran sulfate, poly(G), and poly(I). The question of the possibility of the interaction of the antibodies studied with the charged structures of the cell and that of the role of these antibodies in the normal and pathological states are discussed.
- Research Organization:
- Scientific-Research Institute of Medical Radiology, Obninsk, USSR
- OSTI ID:
- 5629616
- Journal Information:
- Biochemistry (Engl. Transl.); (United States), Vol. 51:4; Other Information: Translated from Biokhimiya; 51: No. 4, 574-578(Apr 1986)
- Country of Publication:
- United States
- Language:
- English
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550201* - Biochemistry- Tracer Techniques