Integration vector for the cyanobacteria Synechocystis sp. 6803

Journal Article · Fri Nov 01 00:00:00 EST 1985 · Dokl. Biol. Sci. (Engl. Transl.); (United States)

OSTI ID:5629031

Shestakov, S V; Elanskaya, I V; Bibikova, M V

The authors have constructed recombinant plasmid DNA molecules with fragments of chromosomal DNA of the cyanobacterium Synechocystis 6803 in the vector plasmid pACYC 184, carrying markers for resistance against tetracycline (TC/sup r/) and chloramphenicol (Cm/sup r/). The authors also present their scheme of constructing integration vectors for Synechocystis 6803. To demonstrate integration of the recombinant plasmids of pSIS and pSIB series into the chromosomes of cyanobacteria, chromosomal DNA was isolated from the Cm/sup r/ transformants of Synechocystis 6803, and used for blot-hybridization using P 32-labeled plasmid DNA of pACYC 184. The hybridization results show that the chromosomal DNA isolated from the Cm/sup r/ transformants of cyanobacteria carries a region homologous with plasmid pACYC 184, whereas the DNA from wild type cells does not hybridize with pACYC 184. The transformation frequency of the Synechocystis 6803 cells by chromosomal DNA from the Cm/sup r/ clones of cyanobacteria was 3-7 x 10/sup -5/, which corresponds to the frequency of chromosomal transformation for other markers.

• Research Organization: Lomonosov Moscow State Univ., Moscow (Russian Federation)
• OSTI ID: 5629031
• Journal Information: Dokl. Biol. Sci. (Engl. Transl.); (United States), Vol. 282:1-6; Other Information: Translated from Dokl. Akad. Nauk SSSR; 282: No. 1, 176-179(May 1985)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHROMOSOMES
GENE RECOMBINATION
CYANOBACTERIA
GENETIC MAPPING
PHOTOSYNTHETIC BACTERIA
PLASMIDS
RECOMBINANT DNA
BIOSYNTHESIS
HYBRIDIZATION
TRACER TECHNIQUES
DNA-CLONING
ELECTROPHORESIS
ENZYMES
GENETICS
LABELLED COMPOUNDS
LABELLING
PHOSPHORUS 32
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
CELL CONSTITUENTS
CLONING
DAYS LIVING RADIOISOTOPES
DNA
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAPPING
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
SYNTHESIS
550401* - Genetics- Tracer Techniques
550301 - Cytology- Tracer Techniques