The effect of UV-B radiation on chloroplast translation in Pisum sativum

Conference · Tue May 01 00:00:00 EDT 1990 · Plant Physiology, Supplement; (USA)

OSTI ID:5628324

Raab, M M; Jagendorf, A T ^[1]

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1. Cornell Univ., Ithaca, NY (USA)

UV-B radiation has previously been reported to reduce growth, flowering, and net photosynthesis. The present study examines the effect of UV-B radiation on isolated chloroplast of 7-10 day old pea seedlings. Amount of ({sup 3}H)-Leu incorporated into isolated chloroplasts was measured in the presence or absence of UV-B exposure. Preliminary experiments show a 30% inhibition of protein synthesis in isolated chloroplasts after only 20 mins of UV-B exposure (6.9 J/m{sup 2}/30 min). Percent inhibition of chloroplast translation is directly correlated with UV-B exposure over a 60 min time span. Preliminary studies also show no change in both cold and radiolabeled protein profiles as expressed on 1-D PAGE and autofluorography. Comparative studies on the sensitivity of e{sup {minus}} flow vs protein synthesis following UV-B exposure are underway. Further work on the role of oxygen free radicals and the specific site of action of UV-B damage to the translation machinery of chloroplasts will be discussed.

• OSTI ID: 5628324
• Report Number(s): CONF-9007196-; CODEN: PPYSA
• Journal Information: Plant Physiology, Supplement; (USA), Vol. 93:1; Conference: Annual meeting of the American Society of Plant Physiologists, Indianapolis, IN (USA), 29 Jul - 2 Aug 1990; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
FAR ULTRAVIOLET RADIATION
BIOLOGICAL EFFECTS
CHLOROPLASTS
PHOTOSYNTHESIS
PISUM
TRACER TECHNIQUES
TRITIUM COMPOUNDS
CELL CONSTITUENTS
CHEMICAL REACTIONS
ELECTROMAGNETIC RADIATION
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LEGUMINOSAE
MAGNOLIOPHYTA
MAGNOLIOPSIDA
PHOTOCHEMICAL REACTIONS
PLANTS
RADIATIONS
SYNTHESIS
ULTRAVIOLET RADIATION
550201* - Biochemistry- Tracer Techniques