2,(3)tert-butyl-4-hydroxyanisole does not reduce SCE induction by benzo(a) pyrene in bone marrow cells of C57BL/6 mice
- Clarion Univ. of Pennsylvania (USA)
Recently, a number of publications have suggested that bone marrow cytogenetics may be used to detect anticarcinogenic or antimutagenic activity. In this work, 0.75% 2(3)-tert-butyl-4-hydroxyanisole (BHA), fed in the diet for 2 weeks, was tested for its ability to reduce the frequency of benzo(a)pyrene (BP)-induced SCE in mouse bone marrow. C57BL/6 male mice were injected i.p. with BP at 0, 33, 67, and 100 mg/kg body weight. There are no significant differences between animals on the control and BHA diets. Excretion of BP in urine over a 72 hr time period was significantly increased in animals on the BHA diet, at both low and high doses. Water-soluble metabolites accounted for all of this increase. It appears that bone marrow is not a good model for the gastrointestinal tract, and that short-term assays for anticarcinogens or antimutagens are more likely to be predictive if they are done in the target organs.
- OSTI ID:
- 5623931
- Journal Information:
- Environmental and Molecular Mutagenesis; (USA), Vol. 16:1; ISSN 0893-6692
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANISOLE
BIOLOGICAL EFFECTS
BENZOPYRENE
MUTAGEN SCREENING
SISTER CHROMATID EXCHANGES
MUTATION FREQUENCY
BONE MARROW CELLS
DIET
MICE
URINE
ANIMAL CELLS
ANIMALS
AROMATICS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CHROMOSOMAL ABERRATIONS
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
ETHERS
HYDROCARBONS
MAMMALS
MATERIALS
MUTATIONS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
RODENTS
SCREENING
SOMATIC CELLS
VERTEBRATES
WASTES
560300* - Chemicals Metabolism & Toxicology