The alternative complement pathway control protein H binds to immune complexes and serves their detection

Journal Article · Sat Jan 01 00:00:00 EST 1983 · Clin. Immunol. Immunopathol.; (United States)

DOI:https://doi.org/10.1016/0090-1229(83)90173-3· OSTI ID:5623380

Nydegger, U E; Corvetta, A; Spaeth, P J; Spycher, M

During solubilization of immune complexes C3b becomes fixed to the immunoglobulin part and serves as a receptor for the alternative complement pathway control protein H. The H-C3b immune complex interaction can be made detectable using 4% polyethyleneglycol to separate free from bound /sup 125/I-H. Tetanus toxoid (Te)/anti-Te complexes kept soluble with fresh serum and containing 125 IU of specific antibody bound 18% of /sup 125/I-H; when fresh serum was chelated with 10 mM EDTA, /sup 125/I-H binding was only 5%. On sucrose density gradients, the H-binding material sedimented in the range of 12 to 30 S. In 36 serum samples from rheumatoid arthritis (RA) patients and in 12 serum samples from patients with systemic lupus erythematosus (SLE), /sup 125/I-H binding was significantly elevated to 9.5 +/- 4.7% (mean +/- 1 SD) and 13.3 +/- 5.6%, respectively, while /sup 125/I-H binding by 36 normal human sera was 4 +/- 2%. RA samples (17/36, 47%) and SLE samples (9/12, 75%) had H-binding values increased by more than 2 SD above the normal mean. The serum samples were also assessed for conglutinin- and C1q-binding activities; a significant correlation between H and C1q binding was observed (P less than 0.001); there was no correlation between H and conglutinin binding. Although binding to immune complexes through its interaction with C3b, H clearly detects a population of complexes other than conglutinin, thus expanding the possibilities of further characterizing pathological complexes.

• Research Organization: Central Laboratory of the Swiss Red Cross, Berne, Switzerland
• OSTI ID: 5623380
• Journal Information: Clin. Immunol. Immunopathol.; (United States), Vol. 26:1
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
IMMUNOGLOBULINS
RADIOIMMUNOASSAY
BLOOD PLASMA
COMPLEMENT
IODINE ISOTOPES
MAN
RHEUMATIC DISEASES
TRACER TECHNIQUES
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
DISEASES
GLOBULINS
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RADIOASSAY
SKELETAL DISEASES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics