Interactions of human lymphoblasts with targeted vesicles containing Sendai virus envelope proteins
- U.A. Centre National de la Recherche Scientifique 530, Montpellier (France)
- USTL Lab. Biologie Physico-Chimique, Montpellier (France)
The authors studied the internalization of targeted fusogenic liposome content to leukemic T cells (CEM) in vitro. They describe a method for the covalent coupling of T101 antibody to the surface of liposomes and the incorporation of fusogenic viral protein into the liposome membrane. Hygromycin B, an impermeant inhibitor of protein synthesis, was encapsulated in the targeted fusogenic liposomes and delivered directly to the cytoplasm of leukemic T cells by fusion between the two membranes. The cytotoxic effect was measured by ({sup 3H})thymidine incorporation. They show that CEM are rapidly and specifically killed by the drug encapsulated in the targeted fusogenic liposomes. This effect is due to the binding of the liposome by means of the antibody and then to the fusion of the liposome with the targeted cell membrane, mediated by F protein.
- OSTI ID:
- 5622937
- Journal Information:
- Experimental Cell Research; (United States), Vol. 185:1; ISSN 0014-4827
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CELL MEMBRANES
CROSS-LINKING
LIPOSOMES
LEUKEMIA
LYMPHOCYTES
PROTEINS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS
ANIMAL CELLS
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DISEASES
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
IMMUNE SYSTEM DISEASES
ISOTOPE APPLICATIONS
LEUKOCYTES
MATERIALS
MEMBRANES
NEOPLASMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
POLYMERIZATION
PYRIMIDINES
RIBOSIDES
SOMATIC CELLS
550301* - Cytology- Tracer Techniques