Interactions of phagocytes with the Lyme disease spirochete: role of the Fc receptor

Journal Article · Mon Oct 01 00:00:00 EDT 1984 · J. Infect. Dis.; (United States)

DOI:https://doi.org/10.1093/infdis/150.4.497· OSTI ID:5617123

Benach, J L; Fleit, H B; Habicht, G S; Coleman, J L; Bosler, E M; Lane, B P

The phagocytic capacity of murine and human mononuclear and polymorphonuclear phagocytes (including peripheral blood monocytes and neutrophils), rabbit and murine peritoneal exudate cells, and the murine macrophage cell line P388D1 against the Lyme disease spirochete was studied. All of these cells were capable of phagocytosing the spirochete; phagocytosis was measured by the uptake of radiolabeled spirochetes, the appearance of immunofluorescent bodies in phagocytic cells, and electron microscopy. Both opsonized and nonopsonized organisms were phagocytosed. The uptake of opsonized organisms by neutrophils was blocked by a monoclonal antibody specific for the Fc receptor and by immune complexes; these findings suggested that most phagocytosis is mediated by the Fc receptor. Similarly, the uptake of opsonized organisms by human monocytes was inhibited by human monomeric IgG1 and by immune complexes. These results illustrate the role of immune phagocytosis of spirochetes in host defense against Lyme disease.

• Research Organization: State of New York Dept. of Health, Stony Brook
• OSTI ID: 5617123
• Journal Information: J. Infect. Dis.; (United States), Vol. 150:4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
RECEPTORS
BIOLOGICAL FUNCTIONS
SPIROCHAETES
LABELLING
PHAGOCYTOSIS
ELECTRON MICROSCOPY
IMMUNITY
IMMUNOGLOBULINS
INHIBITION
MAN
MICE
MONOCLONAL ANTIBODIES
PHAGOCYTES
TRITIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BACTERIA
FUNCTIONS
GLOBULINS
LABELLED COMPOUNDS
MAMMALS
MICROORGANISMS
MICROSCOPY
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RODENTS
SOMATIC CELLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques