Effect of irradiation on the viability of Toxoplasma gondii cysts in tissues of mice and pigs
Muscles from tongue, heart, and limbs of 14 pigs inoculated orally with Toxoplasma gondii oocysts were irradiated with 10, 20, 25, and 30 krad of gamma (cesium-137 and cobalt-60) irradiation. Viability of T gondii cysts was assayed by feeding porcine muscles to T gondii-free cats and/or by inoculation of sediment from acid-pepsin digested porcine muscle into mice. Cats fed 500-g samples of muscles irradiated with up to 20 krad shed T gondii oocysts. Cats fed muscles irradiated with 25 or 30 krad did not shed oocysts. Mice were inoculated with 8 isolates of T gondii, and tissue cysts in their brains irradiated with up to 40 krad were infective to mice; however, there was a 10,000-fold reduction in the viability of organisms in tissue cysts irradiated with 40 krad, compared with that in nonirradiated cysts. At 50 krad of gamma irradiation, there were no detectable infective organisms in infected mouse brains.
- Research Organization:
- Animal Parasitology Institute, Beltsville, MD
- OSTI ID:
- 5616607
- Journal Information:
- Am. J. Vet. Res.; (United States), Vol. 3
- Country of Publication:
- United States
- Language:
- English
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PARASITES
BIOLOGICAL RADIATION EFFECTS
VIABILITY
BRAIN
CESIUM 137
COBALT 60
GAMMA RADIATION
HEART
LIMBS
MICE
MUSCLES
RADIATION DOSES
SWINE
TONGUE
ALKALI METAL ISOTOPES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
BODY AREAS
CARDIOVASCULAR SYSTEM
CENTRAL NERVOUS SYSTEM
CESIUM ISOTOPES
COBALT ISOTOPES
DIGESTIVE SYSTEM
DOMESTIC ANIMALS
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ELECTROMAGNETIC RADIATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MINUTES LIVING RADIOISOTOPES
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORAL CAVITY
ORGANS
RADIATION EFFECTS
RADIATIONS
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560151* - Radiation Effects on Animals- Man