Monitoring of individual human exposure to aflatoxins (AF) and N-nitrosamines (NNO) by immunoassays

Journal Article · Wed Jan 01 00:00:00 EST 1986 · J. Cell. Biochem.; (United States)

DOI:https://doi.org/10.1002/jcb.240300206· OSTI ID:5616563

Wild, C P; Umbenhauer, D; Chapot, B; Montesano, R

Highly sensitive immunoassays have been used to quantitate aflatoxins (AF) and N-nitrosamines (NNO) in human body fluids and tissues, respectively. This approach was taken in order to quantitate environmental exposure to these agents at an individual level to facilitate the investigation of their role in the etiology of human cancer. In order to analyse AF in human urine, an immunopurification step has been developed by using AF-specific antibody bound to AH-Sepharose 4B gel in a small (4-ml gel volume) affinity column prior to enzyme-linked immunosorbent assay (ELISA). The ELISA can be used to quantitate aflatoxin B1 (AFB1) over the range 0.01 ng/ml to 10 ng/ml and the assay system has been validated by using human urine samples spiked with AFB1 over this concentration range. In addition, 29 urine samples from the Philippines have been analyzed and found to contain a range of levels from zero to 4.25 ng/ml AFB1 equivalent with a mean of 0.875 ng/ml. This compared with a mean of 0.066 ng/ml AFB1 equivalent in samples from France. Radioimmunoassay of O6-methyldeoxyguanosine (O6-medG) has been performed on human esophageal and cardiac stomach mucosal DNA from tissue samples obtained during surgery in Linxian County, People's Republic of China, an area of high risk for both esophageal and stomach cancer. Using the methodology described and having 1 mg of hydrolyzed DNA allows the detection of approximately 25 fmol O6medG per mg DNA.

• Research Organization: International Agency for Research on Cancer, Lyon, France
• OSTI ID: 5616563
• Journal Information: J. Cell. Biochem.; (United States), Vol. 2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
AFLATOXIN
CARCINOGEN SCREENING
ESOPHAGUS
NEOPLASMS
ETIOLOGY
NITROSAMINES
STOMACH
BODY FLUIDS
CARCINOGENS
CHINA
COMPARATIVE EVALUATIONS
DNA
DNA REPAIR
ENVIRONMENTAL EXPOSURE
FRANCE
PHILIPPINES
RADIOIMMUNOASSAY
URINE
AMINES
ANTIGENS
ASIA
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOLOGICAL WASTES
BODY
DEVELOPING COUNTRIES
DIGESTIVE SYSTEM
DISEASES
EUROPE
GASTROINTESTINAL TRACT
IMMUNOASSAY
IMMUNOLOGY
ISLANDS
ISOTOPE APPLICATIONS
MATERIALS
NITROSO COMPOUNDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
RADIOASSAY
RADIOIMMUNOLOGY
RECOVERY
REPAIR
SCREENING
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
WASTES
WESTERN EUROPE
560306* - Chemicals Metabolism & Toxicology- Man- (-1987)
550901 - Pathology- Tracer Techniques