Encapsulation of thiosulfate: cyanide sulfurtransferase by mouse erythrocytes

Journal Article · Sun Mar 30 00:00:00 EST 1986 · Toxicol. Appl. Pharmacol.; (United States)

DOI:https://doi.org/10.1016/0041-008X(86)90327-3· OSTI ID:5615382

Leung, P; Ray, L E; Sander, C; Way, J L; Sylvester, D M; Way, J L

Murine carrier erythrocytes, prepared by hypotonic dialysis, were employed in the encapsulation of several compounds including (14C)sucrose, (3H)inulin, and bovine thiosulfate:cyanide sulfurtransferase (rhodanese), a mitochondrial enzyme which converts cyanide to thiocyanate. Approximately 30% of the added (14C)sucrose, (3H)inulin, and rhodanese was encapsulated by predialyzed erythrocytes, and a decrease in the mean corpuscular volume and mean corpuscular hemoglobin was observed. In the encapsulation of rhodanese a recovery of 95% of the erythrocytes was achieved and an 85% equilibrium was established. The addition of potassium cyanide (50 mM) to intact, rhodanese-loaded erythrocytes containing sodium thiosulfate resulted in its metabolism to thiocyanate. These results establish the potential use of erythrocytes as biodegradable drug carrier in drug antagonism.

• Research Organization: Texas A and M Univ., College Station
• OSTI ID: 5615382
• Journal Information: Toxicol. Appl. Pharmacol.; (United States), Vol. 1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
THIOSULFATES
ENCAPSULATION
TRANSFERASES
CARBON 14 COMPOUNDS
CARRIERS
CYANIDES
DIALYSIS
DRUGS
ERYTHROCYTES
INULIN
METABOLISM
MICE
SACCHARIDES
TRITIUM COMPOUNDS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
ENZYMES
LABELLED COMPOUNDS
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
POLYSACCHARIDES
RODENTS
SEPARATION PROCESSES
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)