Hydrogen-1, carbon-13, and nitrogen-15 NMR spectroscopy of Anabaena 7120 flavodoxin: Assignment of. beta. -sheet and flavin binding site resonances and analysis of protein-flavin interactions

Journal Article · Mon Oct 01 00:00:00 EDT 1990 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00493a014· OSTI ID:5615095

Stockman, B J; Krezel, A M; Markley, J L ^[1]; Leonhardt, K G; Straus, N A ^[2]

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1. Univ. of Wisconsin, Madison (USA)
2. Univ. of Toronto, Ontario (Canada)

Sequence-specific {sup 1}H and {sup 13}C NMR assignments have been made for residues that form the five-stranded parallel {beta}-sheet and the flavin mononucleotide (FMN) binding site of oxidized Anabaena 7120 flavodoxin. Interstrand nuclear Overhauser enhancements (NOEs) indicate that the {beta}-sheet arrangement is similar to that observed in the crystal structure of the 70% homologous long-chain flavodoxin from Anacystis nidulans. A total of 62 NOEs were identified: 8 between protons of bound FMN, 29 between protons of the protein in the flavin binding site, and 25 between protons of bound FMN and protons of the protein. These constraints were used to determine the localized solution structure of the FMN binding site. The electronic environment and conformation of the protein-bound flavin isoalloxazine ring were investigated by determining {sup 13}C-{sup 1}H coupling constants. The carbonyl edge of the flavin ring was found to be slightly polarized. The xylene ring was found to be nonplanar. Tyrosine 94, located adjacent to the flavin isoalloxazine ring, was shown to have a hindered aromatic ring flip rate.

• OSTI ID: 5615095
• Journal Information: Biochemistry; (USA), Vol. 29:41; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
FLAVINES
NUCLEAR MAGNETIC RESONANCE
ALGAE
CARBON 13
CHEMICAL SHIFT
COENZYMES
HEAVY WATER
HYDROGEN 1
NITROGEN 15
NMR SPECTRA
OVERHAUSER EFFECT
PROTEINS
SPIN
ACRIDINES
AMINES
ANGULAR MOMENTUM
AROMATICS
AZAARENES
AZINES
CARBON ISOTOPES
EVEN-ODD NUCLEI
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROGEN ISOTOPES
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
NITROGEN ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PARTICLE PROPERTIES
PLANTS
PYRIDINES
RESONANCE
SPECTRA
STABLE ISOTOPES
WATER
550201* - Biochemistry- Tracer Techniques