A novel minimum ribozyme with oxidoreduction activity
- Mitsubishi Kasei Institute of Life Sciences, Tokyo (Japan)
- Tohoku Univ., Sendai (Japan)
A nucleoside catalyzing the oxidoreduction of NADH and K{sub 3}Fe(CN){sub 6} was isolated from Torula yeast RNA and also obtained in 0.05{percent} yield by a series of steps: SDS-phenol extraction, nuclease P{sub 1} digestion, alkaline phosphatase digestion, anion exchange chromatography, and HPLC on an ODS column. Its chemical structure was clearly determined at 5-hydroxycytidine, from the results of FAB-MS and {sup 1}H and {sup 13}C NMR spectroscopies. The mass spectra, chromatographic behavior, UV spectra, and NMR spectra of this nucleoside from natural and synthetic sources were identical. This is the first report of an RNA catalyst having catalytic activity except for the cleavage and ligation of phosphodiester bonds of RNA. That an RNA has oxidoreduction activity indicates new possibilities for RNAs as living molecules. 5-hydroxycytidine may be a vestige of RNAs that formerly possessed metabolizing ability.
- OSTI ID:
- 5613578
- Journal Information:
- Biochemistry; (USA), Vol. 29:47; ISSN 0006-2960
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
NUCLEOSIDES
NUCLEAR MAGNETIC RESONANCE
RNA
BIOCHEMICAL REACTION KINETICS
CARBON 13
CYTIDINE
ENZYME ACTIVITY
HYDROXY COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MASS SPECTRA
NMR SPECTRA
PROTONS
TORULA
AZINES
BARYONS
CARBON ISOTOPES
CHROMATOGRAPHY
ELEMENTARY PARTICLES
EUMYCOTA
EVEN-ODD NUCLEI
FERMIONS
FUNGI
HADRONS
HETEROCYCLIC COMPOUNDS
ISOTOPES
KINETICS
LIGHT NUCLEI
MAGNETIC RESONANCE
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
NUCLEONS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANTS
PYRIMIDINES
REACTION KINETICS
RESONANCE
RIBOSIDES
SEPARATION PROCESSES
SPECTRA
STABLE ISOTOPES
YEASTS
550601* - Medicine- Unsealed Radionuclides in Diagnostics