In vitro conditions modify immunoassayability of bovine pituitary prolactin and growth hormone: insights into their secretory granule storage forms
The amount of immunoassayable intracellular bovine (b) PRL and GH varies depending on treatment conditions. The present studies were designed to characterize the mechanisms involved and to compare immunoassayability of both hormones under similar conditions. Pituitary homogenate and secretory granule hormones displayed both time- and temperature-dependent increases when incubated at pH 10.5 with reduced glutathione. Changes in immunoassayability seem to reflect conversion from poorly immunoactive tissue hormone oligomers to monomeric hormone. The data indicate that oligomeric bPRL is stabilized primarily by intermolecular disulfide bonds, although it is also susceptible to urea, SDS, and EDTA; granule thiols may also influence the conversion to monomer. The storage form of bGH appears to be stabilized differently. Maneuvers demonstrated in these studies to influence immunoassayability correlate very well with their previously established effects on hormone release and secretion, strengthening the likelihood that a functional link exists between assayability and secretion.
- Research Organization:
- Univ. of Rochester School of Medicine and Dentistry, NY
- OSTI ID:
- 5612363
- Journal Information:
- Endocrinology; (United States), Vol. 116:4
- Country of Publication:
- United States
- Language:
- English
Similar Records
Depletion of bovine pituitary prolactin by cysteamine involves a thiol:disulfide mechanism
Cysteamine inhibition of prolactin immunoassayability and secretion: studies with aminothiophenols and other analogs
Related Subjects
LTH
RADIOIMMUNOASSAY
COMPARATIVE EVALUATIONS
STH
COWS
GLUTATHIONE
IN VITRO
MEA
PH VALUE
PITUITARY GLAND
SODIUM COMPOUNDS
THIOLS
ALKALI METAL COMPOUNDS
AMINES
ANIMALS
BODY
CATTLE
DOMESTIC ANIMALS
DRUGS
ENDOCRINE GLANDS
GLANDS
GONADOTROPINS
HORMONES
ISOTOPE APPLICATIONS
MAMMALS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PEPTIDE HORMONES
PEPTIDES
PITUITARY HORMONES
POLYPEPTIDES
PROTEINS
RADIOASSAY
RADIOPROTECTIVE SUBSTANCES
RUMINANTS
TRACER TECHNIQUES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics