Free lung cell phagocytosis and the effect of cigarette smoke exposure
Abstract
We report on a technique for studying phagocytosis in free lung cells with the use of fungal spores. Free lung cells were obtained from a bronchial lavage. They were incubated with fungal spores and the engulfment of these spores was studied at various time intervals and under different conditions. The phagocytosis process was found to occur from relatively stationary macrophages within the first hours after incubation. The number of engulfed spores was proportional to their number in the solution. Addition of serum or surfactant to the medium increased the phagocytosis rate. In hamsters and rats exposed to tobacco smoke under in vivo conditions, a dose-related increase in phagocytosis rate could be demonstrated.
- Authors:
- Publication Date:
- OSTI Identifier:
- 5611015
- Resource Type:
- Journal Article
- Journal Name:
- Exp. Lung Res.; (United States)
- Additional Journal Information:
- Journal Volume: 1:2
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; RESPIRATORY TRACT CELLS; SENSITIVITY; TOBACCO SMOKES; BIOLOGICAL EFFECTS; FUNGI; HAMSTERS; MACROPHAGES; PHAGOCYTOSIS; RATS; SPORES; AEROSOLS; ANIMAL CELLS; ANIMALS; COLLOIDS; CONNECTIVE TISSUE CELLS; DISPERSIONS; MAMMALS; PHAGOCYTES; PLANTS; RESIDUES; RODENTS; SMOKES; SOLS; SOMATIC CELLS; VERTEBRATES; 560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987); 551000 - Physiological Systems; 550300 - Cytology
Citation Formats
Fogelmark, B, Rylander, R, Sjoestrand, M, and Reininghaus, W. Free lung cell phagocytosis and the effect of cigarette smoke exposure. United States: N. p., 1980.
Web. doi:10.3109/01902148009069643.
Fogelmark, B, Rylander, R, Sjoestrand, M, & Reininghaus, W. Free lung cell phagocytosis and the effect of cigarette smoke exposure. United States. https://doi.org/10.3109/01902148009069643
Fogelmark, B, Rylander, R, Sjoestrand, M, and Reininghaus, W. 1980.
"Free lung cell phagocytosis and the effect of cigarette smoke exposure". United States. https://doi.org/10.3109/01902148009069643.
@article{osti_5611015,
title = {Free lung cell phagocytosis and the effect of cigarette smoke exposure},
author = {Fogelmark, B and Rylander, R and Sjoestrand, M and Reininghaus, W},
abstractNote = {We report on a technique for studying phagocytosis in free lung cells with the use of fungal spores. Free lung cells were obtained from a bronchial lavage. They were incubated with fungal spores and the engulfment of these spores was studied at various time intervals and under different conditions. The phagocytosis process was found to occur from relatively stationary macrophages within the first hours after incubation. The number of engulfed spores was proportional to their number in the solution. Addition of serum or surfactant to the medium increased the phagocytosis rate. In hamsters and rats exposed to tobacco smoke under in vivo conditions, a dose-related increase in phagocytosis rate could be demonstrated.},
doi = {10.3109/01902148009069643},
url = {https://www.osti.gov/biblio/5611015},
journal = {Exp. Lung Res.; (United States)},
number = ,
volume = 1:2,
place = {United States},
year = {Sun Jun 01 00:00:00 EDT 1980},
month = {Sun Jun 01 00:00:00 EDT 1980}
}
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