Scavenging of hydrogen peroxide by alveolar type II pneumocytes (ATII) and macrophages (MAC)

Conference · Mon Feb 26 00:00:00 EST 1990 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5610697

Easterling, L; Slater, M; Baker, R; Matalon, S ^[1]

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1. Univ. of Alabama, Birmingham (United States)

The pulmonary epithelium is often a target of increased concentrations of extracellularly generated reactive oxygen species (PROS). In this study the authors quantified whether freshly isolated ATII and unstimulated MAC may scavenge extracellularly generated hydrogen peroxide (H{sub 2}O{sub 2}) and to determine the mechanisms involved. Cells were suspended in Eagles minimum essential medium and incubated with 500 {mu}M xanthine and 10 mU/ml xanthine oxidase for 60 min at 37C. In a separate series of experiments cells were preincubated with 10 {mu}M aminotriazole (ATZ) which decreased their catalase activity 60% of their initial values. Both ATII and MAC scavenged significant amounts of H{sub 2}O{sub 2}. After exposure to H{sub 2}O{sub 2} cell viabilities for the control (-ATZ) and ATZ treated cells were {ge}50%. The ability of ATII and MAC to scavenge extracellular H{sub 2}O{sub 2} may protect the alveolar epithelium from reactive oxygen species injury.

• OSTI ID: 5610697
• Report Number(s): CONF-9004153-; CODEN: FAJOE
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 4:3; Conference: 74. annual meeting of the Federation of American Societies for Experimental Biology, Washington, DC (United States), 1-5 Apr 1990; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
HYDROGEN PEROXIDE
SCAVENGING
MACROPHAGES
BIOLOGICAL FUNCTIONS
ANIMAL CELLS
CATALASE
ENZYME ACTIVITY
EPITHELIUM
LUNGS
OXIDOREDUCTASES
VIABILITY
XANTHINES
ANIMAL TISSUES
AROMATICS
AZAARENES
BODY
CONNECTIVE TISSUE CELLS
ENZYMES
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PEROXIDASES
PEROXIDES
PHAGOCYTES
PROTEINS
PURINES
RESPIRATORY SYSTEM
SOMATIC CELLS
TISSUES
560300* - Chemicals Metabolism & Toxicology